DopGE : METHODS OF CULTURE OF ASCOBOLACEAE 175 
conidium is very close. Whether fusion takes place can not be 
determined from the preparations as studied in glycerin jelly. 
There can be no question, however, as to the specific attraction 
between the trichogyne and the conidium, and I shall call it the 
antheridial conidium. 
We may now note some variations from this type of develop- 
ment. FiG. 36 shows an archicarp in which the stalk is evenly 
coiled in a sort of snail-shell form. The conidium giving rise to 
the coil is concealed below but the stalk of the conidium is evi- 
dently at b. The ascogonium has made only one or two turns 
before growing far out in the medium. The outer portion has 
coiled upon itself and either come to an end at j or continued 
as a hyaline filament (2) which has come in contact with a mycelial 
hypha. This figure was drawn from the first fruit of this species 
that I had seen, and before the existence of the long transparent 
trichogyne was known. Since investing hyphae were being formed 
about the ascogonium, fertilization had evidently already taken 
place and the trichogyne, being dead, would scarcely have been 
visible without staining. 
FIG. 34 shows an archicarp that has failed to develop further, 
probably Owing to some injury received when an adjacent portion 
of the medium was removed. The first part of the stalk coil 
could not be found, and the remaining cells are entirely empty. 
The other regions, however, are very plainly marked, especially 
the connection between the stalk coil and the first cell of the 
ascogonium. - The end of th trichogyne is plainly coiled around 
an antheridial conidium. ° 
tis ids . germinate in liquid extract of heated soil and form 
archicar Soll ey I have never been able to find the complete 
of etal a liquid medium. Occasionally one finds such cases 
that af development in cultures on agar media. Fic. 42 shows 
alter the stalk coil has been formed, it may take on a 
th. 
Vegetative grow 
