DopGE: METHODS OF CULTURE OF ASCOBOLACEAE 179 
into the medium. When this occurs, one frequently finds that it 
gives rise to a slender branch at about the fourth”cell. This side 
branch (FIG. 17, d) may in some cases curve over and come in 
contact with a hypha arising from the first cell of the stalk of the 
ascogonium, but I am unable to state at present whether this is 
a constant feature in the process of development. None of the 
cultures from the germinated spores has produced ascocarps. 
Ternetz has proved that Ascophanus carneus does not produce 
fruit when kept in darkness. My cultures were placed in a very 
dimly lighted room and this may account for their failure to pro- 
duce ascocarps. Under the same conditions, however, Ascobolus 
Winteri fruited abundantly. 
AscopoLus WINTERI Rehm: Rabenhorst, Krypt.-Fl. 1%: 1124. 
1896. 
The identification of this species was a matter of some difficulty. 
In some features it resembles Ascobolus brunneus Cooke, A. 
Stictoideus Speg., A. amoenus Oud., A. Leveillei (Cur.) Boud., and 
‘A. Leveillei var. americanus Cooke & Ellis. The spores from 
apothecia grown in a vasculum are smooth, hyaline, and about 30” 
length. They appear to be perfectly mature. The asci project 
from the ascocarp, and the spores are shot upward several centi- 
meters. Such spores might be those of an Ascophanus. Spores 
in the asci from cultures in the light are pale amethyst colored. 
It is Possible to so place this species with reference to the light that 
hyaline spores will be thrown off from apothecia on one edge of 
the substratum and well colored spores from the other. The 
Spores are delicately warted; the warts being arranged in short 
broken lines give it a somewhat reticulated appearance, a char- 
acteristic of the spore of A. brunneus according to Rehm (1896). 
A few narrow cleftlike furrows in the epispore are visible (FIG. 12). 
ba @pparent clefts resemble those peculiar to the spores of A. 
pe om ; (Fic. 20). ne young apothecia may appear to be 
thas 3 a. greenish, or cinereous, but this color is probably only 
Sg the substratum showing through. _The apothecia are 
Pe Shenae and colorless. Ch. A. stictoideus Speg. The 
ecies Could not be identified with the descriptions of any known 
I ee ; . : 
n the Massee collection is a specimen accompanied with 
