DopcE: METHODS OF CULTURE OF ASCOBOLACEAE 18] 
may remain entirely free from apothecia. When spores are sown 
at two points on opposite sides of the plate rather close to its edge, 
the hyphae from either region will not cross over into the territory 
of the other, but the apothecia are formed in an irregular line 
just back of the tips of the hyphae, parallel to a neutral zone 
between the two mycelia. (TExtT FIG. I.) 
3 2 
Fic. 1. Shows the growth of the mycelium and formation of the apothecia of 
Ascobolus Winteri in a culture inoculated at two points on opposite sides of the plate. 
Pio. A large number of apothecia are formed in the region at c, where a 
colony of bacteria has developed. 
The behavior of the vegetative hyphae with reference to 
bacterial colonies present in the cultures, also shows some interest- 
Mg peculiarities. In some cases the mycelial hyphae would run 
through and Over the bacterial colonies and mingle with them. 
Tn other cases the growth of the hyphae was checked at some dis- 
tance from the bacterial colony, apparently owing to the emanation 
of toxic substances from it. A sterile zone one centimeter wide 
Was sometimes left between the hyphae and the bacteria. 
Bordering this zone a dense aggregation of apothecia would form. 
TEXT FIG. 2.) 
No circular concentric zones of fruit bodies were formed in this 
€s. Any factor tending to limit the vegetative growth of the 
Mycelium serves to bring about the formation of apothecia. 
The manner in which the mycelium made its way in a hard 
medium deserves notice. Ordinarily the mycelium grows along 
