DopGE: METHODS OF CULTURE OF ASCOBOLACEAE 185 
parent vegetative hyphae. The archicarp consists of about 
twenty cells, of which we may say four to six belong to the stalk, 
about eight to the ascogonial region, and the remaining cells to 
the trichogyne. A large number of archicarps were stained in 
toto with aceto-carmin and iron-hematoxylin. Ina majority of 
these preparations there were three or four cells of the archicarp 
that took but little stain. All the other cells contained several 
nuclei while only an occasional nucleus could be found in the 
region of these hyaline cells (FIG. 25). I have seen no cultures 
that appear to be more vigorous that this one, and as there were 
many apothecia in all stages of development present, I can not 
believe this was a pathological condition. If such were the case 
it was pretty generally distributed in the culture. 
In this species as in all others I have studied, the spiral nature 
of the coil is much altered as the ascocarp is developed. The 
investing hyphae push in between the turns and straighten out 
the coil so that sections of the apothecium show a wormlike 
y. 
The young apothecia are covered with a secondary mycelium 
which spreads out in all directions, even directly upward to the 
Surface of the medium when the apothecium is completely im- 
bedded. The time required for the production of the mature 
fruit seems to be much longer in these artificial cultures than is 
the case with plants developing on the natural substratum. 
Ascogo.us FURFURACEUs Pers. 
The manner in which the spores germinate (FIG. 29) does not 
differ materially from that described for other species. Nine days 
after the germination, the cultures contained hundreds of archi- 
carps and young ascocarps. The light greenish color was present 
at a very early stage. Molliard (1903) and Claussen (1905) found 
sreat numbers of oidia were produced on the mycelium. As has 
been poted above, the former was unable to obtain ascocarps 
ee the introduction of bacteria. Claussen grew the myce- 
frets S Oidia for one hundred generations without obtaining 
cultures am very certain that no oidia were produced in these 
a ’ NOY were there any chlamydospores such as Welsford 
997) has described. These asexual spores may possibly be 
