DopGE : METHODs OF CULTURE OF ASCOBOLACEAE 193 
6. The archicarps of Ascophanus carneus, Ascobolus immersus, 
A. furfuraceus, and A. Winteri arise directly from the mycelium; 
they are spirally coiled organs of which the peripheral cells rep- 
resent a more or less strongly developed trichogyne. The tricho- 
gyne frequently becomes attached to a hypha growing out at the 
base of the archicarp. 
7. The general character of the archicarps described and the 
presence of septate trichogynes, support the view that the lichens 
tepresent primitive forms of the Ascomycetes. 
OLUMBIA UNIVERSITY. 
Explanation of plates 10-15 
The magnifications are given in connection with each figure. In some cases the 
surface markings of the spores are not shown in the figure. 
PLATE I0 
ig. I. a, germinated spore of Thecotheus Pelletieri, unstained. 275. 
6, germinated spore of Thecotheus Pelletieri, showing germination from only one germ 
pore, stained in toto. X425. 
Fic. 2, 3. germinated spores of Ascobolus viridis var. 450. 
Fic. 4. a, immature spore of A. viridis var. X800. 6, mature spore of A. 
viridis var, 800 
Fic. 5. Very large spore of A. viridis Curr. from no. 196 Phil. Elv. Brit. 800. 
Fic. 6. Germinated spore of Saccobolus neglectus, showing the manner in which 
the epispore has been broken away during germination. 450. 
Fic. 7. Group of eight spores of Saccobolus neglectus, in which one spore has 
serminated and a second spore (a) has swollen preparatory to germination. 450. 
Fic. 8; Spores of Ascobolus pusillus Boud. 00, 
1G. 9. Ascobolus carbonarius: a, germinated spore; b, swollen vesicle on the 
hypha; c, conidium; X275; d, another vesicle stained with aceto-car carmin; X450. 
Fic. 10, Conidium of A. carbon narius, showing swollen protuberance. 250. 
Fic. rr, Spore of A. carbonarius, showing the ends capped with a thickening 
of the epispore. 800 
Fic. 12, Spores of Ascobolus Winteri. X 700. 
Fic. 13. Germinated spores of A. Winteri. x 
Fic. 14. Mycelium and germinated spore of A. Winter X725 
FIG. 35. Two spores of — neo one ee germinated. X450. 
? Fic, 16. Germin ated spore . g in which the 
Pispore becomes cracked due to ee X400. 
Hi ai d, hypha in contact with the ‘icreas: 250. 
. 18. Germinated spore of A. carne 8 
ia T9 Germinated spores of Ascophanus sarcobius. X380 
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