194. DopcE: METHODS OF CULTURE OF ASCOBOLACEAE 
Fic. 21. Germinated spore of A. immersus. X250 
Fic. 22, 23. Germinated spores and mycelium of A immersus. 650. 
Fic. 24. Archicarp of A. immersus, stained in toto with aceto-carmin: a, stalk 
cells; b, ascogonium; ¢, trichogyne torn from the hypha (d). 630. 
chicarp of A. immersus stained with aceto-carmin, showing four 
a SOE empty cells. 630. 
Fic. 26. Spores of Ascobolus glaber: a shows the characteristic markings of a 
mature spore; J, eereiantet spore. ><900 
. 27. Germinated spores of adiiniies Leveillet. 650. 
Fic. 28. aus of Ascobolus furfuraceus: a, stalk cells; b, ascogonium; 
c, trichogyne. 50. 
Fic. 29. Spores of A. furfuraceus: a, b, ungerminated spores; ¢, d, germinated 
spores. 360 
PLATE 12 
Ascobolus carbonarius 
The lettering for each figure is the same as given for FIG. 33. 
Fic. 30. Section of a germinated spore of A. carbon arius. 650. 
Fic. 31. Germinated spore of A. carbonarius: a, optical section of a mature 
spore; 6, immature spores which germinated. 0. 
Fic. 32. a, section of a conidium of A. POE b, sections of germinated 
female conidia. 650. : 
Fic. 33. Mature archicarp: a, conidium giving rise to the stalk coil; b, conidio- 
phore; ¢, hypha arising from conidiophore; d, first cell of stalk coil (f); g, last cell ot 
stalk coil giving rise to the ascogonium (h); j, last cell of ascogonium; k, first cell of 
trichogyne (J); m, male conidium or antheridium (?); n, stalk of antheridial conidium. 
400. 
Fic. 34. Archicarp in a pathological condition. 400 
Fic. 35. Archicarp in which the trichogyne (J) does not Sil about the conidium 
(m), which is entirely pn Ris: 
Fic. 36. Archi ae the characteristic trichogyne was not seem; : 
may be the trichogyne. me 
. Fic. 37. Archicarp in Bre the trichogyne lies beneath; m, a conidium near 
Yy. ‘ 
Fic. 38. Cells of the ascogonium giving rise to primary ascogenous hyphae: 
X 400. 
4: Peet nee ae ‘ oer ae ascogenous hyphae 
Wildl pi iliicss y 
Fic. 39. 
o, Fe A00, 
PLATE 13 
Ascobolus carbonarius 
Lettering as in FIG. 33. 
FIG. Mature archicarp showing the trichogyne (J) coiled twice 
conidium (m). 300. 
Fic. 41. Archicarp becoming invested with hyphae: a trichogyne is 
l, the remaining portion being concealed beneath the ascogonium. 300 has 
Fic. 42. The conidium (a) has germinated, giving rise to the stalk coil | which 
begun vegetative growth; b, the conidiophore; c, hypha arising from the conidiopho 
about the 
visible at 
FIG. 43. a, germinated conidium with short thick stalk (6). 30% 
