SKINNER AND BEATTIE: EFFECT OF ASPARAGIN ON WHEAT 401 
found it was beneficial to Lemna; Brown* found it beneficial to 
the barley embryo; Nakamura? secured beneficial results, working 
with the barley plant, and also found it beneficial to onions; and 
Molliard f secured beneficial results with radish. All of these 
tests were made in water cultures. 
EXPERIMENTAL METHODS 
In studying the effect of asparagin on growth, wheat seedlings 
were grown in aqueous culture solutions containing the nutrient 
salts, calcium acid phosphate, sodium nitrate, and potassium sul- 
phate. Some of the cultures contained each of the salts singly, 
others were composed of a mixture of two salts, sodium nitrate 
and calcium acid phosphate, sodium nitrate and potassium sul- 
phate, and calcium acid phosphate and potassium sulphate. Still 
other solutions had all three constituents in various proportions. 
Inall there were 66 different cultures of nutrient solutions. The 
scheme of the experiment and manner of preparing the nutrient 
cultures was similar to that described in Bulletin 70 of the Bureau of 
Soils and other publications.§ The concentration of all these solu- 
ons was 80 parts per million of the fertilizer ingredients, PsO;, NHs, 
and KO. In cultures containing only one nutrient salt, for in- 
Stance calcium acid phosphate, the concentration was 80 parts per 
million of P,O;. If two salts were present, for instance calcium 
acid Phosphate and sodium nitrate, the concentration was 80 
Parts per million of P,Q; + NHs. If all three salts were present 
the concentration was 80 parts per million of P2O; + NH: + K,0. 
The ratios of these concentrations varied in 10 per cent stages, mak- 
“8 inall 66 different cultures. Distilled water treated with carbon 
Was used in preparing the culture solutions. The culture solutions 
— acaba as On the culture of excised embryos of barley on nutrient solu- 
1906, taining nitrogen in different forms. Trans. Guinness Research Lab. 1: 288. 
nay a ura, T. Relative value of asparagin as a nutrient for phanerogams. 
Agr. Tokyo 2: 465. 1894. 
"ee M. Recherches sur l'utilisation par les plantes supérieures de 
ca organiques azotées. Bull. Soc. Bot. France IV. 10: 541. 1910. 
Some effects of a harmful organic soil con- 
he - Dept. Agr. Bur. Soils Bull. 70: 1-98. 1910; Bot. Gaz. 50: 161. 
Gaz. 50: npg’ nitrate and potassium on absorption and growth. Bot. 
ee To. 
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