SKINNER AND BEATTIE: EFFECT OF ASPARAGIN ON WHEAT 433 
taken at the termination of the experiment, are given in TABLE I. 
The first column gives the number of the culture; the second, third, 
and fourth the amount of the fertilizer ingredient in the culture 
solution. Calcium acid phosphate, sodium nitrate, and potassium 
sulphate were the nutrient salts used.. The fifth column gives the 
green weight of the plant without asparagin, and the sixth column 
the green weight with 50 parts per million of asparagin in the 
solution. 
TABLE I 
EFFECT OF ASPARAGIN ON GROWTH IN CULTURE SOLUTIONS CONTAINING NO NITRATE 
Fertilizer ingredients in culture solution Green weight of cultures 
Ree. rc Ot eee ee eae soe 
P20;, parts per | NHs, parts per | KO, parts per | Without a: pare With 50 p- p.m. 
mhillion million million | gin, grams asparagin, gms. 
Seer ec } ER 
56 ) oO 80 Pi eRe 1.832 
46 8 re) 72 | 1.368 1.644 
37 16 te) 64 1.382 1.742 
hd 24 Ie 56 1.119 1.825 
oad 32 cs) 48 1.393 2.145 
16 40 oO 40 1.223 2.199 
II | 48 re) 32 I.167 1.932 
7 56 oO 24 1.243 2.199 
4 64 0 16 1.313 1.909 
3 72 (9) 8 I.311 1.775 
I 80 oO ° 0.811 0.975 
All of the cultures with asparagin present show a marked in- 
‘rease in weight. Culture no. 37 containing 16 parts per million 
of Phosphate and 64 parts per million of potash gave 1.382 grams 
sreen weight without asparagin and 1.742 grams with asparagin; 
culture no. 16, which contained 40 parts per million both of phos- 
phate and of potash gave 1.223 grams weight without asparagin and 
2.199 grams with asparagin. Culture no. 4, composed of 64 parts 
Per million Phosphate and 16 parts per million potash, gave 1.313 
sams green weight without asparagin and 1.909 with asparagin. 
The total weight of the 11 cultures without asparagin was 13.714 
sams against 20.478 grams with asparagin, thus showing an in- 
‘Tease of 47 per cent as an average effect in the various cultures 
Without Nitrate. This series of cultures is reproduced in PLATE 33. 
“ultures marked with the same number, for instance 56 and 564, 
| ve the same Proportion of potash and phosphate. Cultures 
; “aked with the number alone contain no asparagin, the cultures 
| with me letter A have 50 parts per million of asparagin. The 
—“Mposition of the culture solution is given in TABLE I. It will 
