ne a ee ee ee es) a ee a ae 
ANDREWS: PROTOPLASMIC STREAMING IN MucoR 457 
fer and some of M. Mucedo that have kept for two years, and most 
of them still grow quite as well as the spores of these plants that 
are only a few days old. They have been preserved in a dry condi- 
tion on bread, on which they grew in wide-mouthed bottles closed 
with cotton. This is also a very convenient way in which one may 
preserve the spores of those plants it is desired to grow—when 
first it is ascertained that the culture sealed in the bottle is a pure 
one. One may then by means of a sterilized needle or forceps 
easily remove a few spores to the desired culture media and in 
this way by a great saving of time and labor obtain pure cultures 
for study. Schréter* used Phycomyces nitens and Mucor stolonifer 
for his study; Arthur} used Rhizopus nigricans; de Vries,t Phy- 
comyces nitens; Ternetz,§ Ascophanus carneus; and Woronin,]|| 
Ascobolus pulcherrimus. Of the three forms that I used, viz: 
Mucor stolonifer, M. Mucedo, and to some extent Phycomyces nitens, 
I found M. Mucedo also to be easily obtainable and as well 
adapted to the investigations of this paper as M. stolonifer. In 
addition, then, to my work on the other two forms my experiments 
with Mucor Mucedo, made to confirm or disprove Schréter’s results, 
will also show whether or not the phenomena he and Ternetz 
describe are observable in still another fungus to the same extent 
as he mentions for M. stolonifer. 
APPARATUS AND METHOD 
A description of the methods used in working out this paper 
fan not all be described in one place, but the methods used in 
the various experiments can be materially shortened by describing 
and figuring at the outset a few of the principal pieces of apparatus 
used, to which reference may be made in the study where they 
were employed. For this purpose FIG. 1 is an illustration of the 
*Pparatus used. In some of the earlier experiments the apparatus 
in FIG. I was used in a somewhat more simplified form, but 
t De Vries, H. Bot. Zeit. 44: 1-6. 1885. 
§ Ternetz, loc. cit. 273-309. 
ll Ternetz, loc. cit. 273-3009. 
| Schroter, loc, cit. 10-20. 
