464 ANDREWS: PROTOPLASMIC STREAMING IN MucoR 
EXPERIMENTAL PART 
The investigations were begun by a series of experiments 
to ascertain what culture media were best for the growth of these 
fungi. The specimens of Mucor stolonifer and M. Mucedo were 
grown in various nutrient media, which require a word of explana- 
tion here. Some of the spores of Mucor Mucedo, M. stolonifer, and 
Phycomyces nitens were observed to germinate very well in a 5 
per cent solution of pure cane sugar. Other concentrations of the 
cane sugar solutions were used, but the 5 per cent solutions gave 
excellent results and under favorable conditions of temperature 
etc. a rapid growth resulted. In some experiments they grew 
quite well for a time in water, but this did not suffice for the 
conditions which the experiments of this study made necessary. 
Also a favorable medium was formed for the growth of the fungi 
in a tolerably concentrated solution of plum juice obtained in a 
suitable condition by filtering first through a thin layer of cheese- 
cloth and then through filter paper. A solution made from horse 
manure caused a good growth, but it was inferior in this respect 
to sugar or plum solution, as was also a solution made by adding 
a little glycerin to pure water. 
Another good medium for the growth of these fungi is bouillon 
made as follows: 
50 grams lean beef, 
100 c.c. water. 
Let this stand from one to two hours at 50° C., then filter 
through cheesecloth. If less than 100 c.c. filter through add 
water to make that volume. Add 1 gram dry peptone and 0-5 
gram of common salt, then boil, neutralize, clear, and filter. 
In some experiments gelatin was used, made as follows: 
50 grams lean beef, 
gelatin 10 grams, 
dry peptone I gram, 
salt 0.5 gram. 
Heat, neutralize, clear, and filter. 
Agar-agar was also used. It was made by adding 15 gram : 
of finely chopped agar-agar to the bouillon solution above sani 
tioned. A concentrated solution of very saccharin grape 1 
was also a good medium for fungal growth. 
