466 ANDREWS: PROTOPLASMIC STREAMING IN Mucor 
has found that the nature of the nutrient medium was of great 
influence in causing a very large growth of fungal filaments where 
sugar was used. But this he found not to be the case for all sorts 
of sugar. He also found that the same applies to peptone solutions 
of certain concentrations. It has therefore been of importance in 
this study to select those culture media that will cause a rapid 
growth of fungal hyphae having large diameter so as to carry 
on the investigation to better advantage. The gelatin solution 
grew fungal hyphae of very large diameter and conspicuous stream- 
ing movements. The nutrient media, then, in some cases pro- 
duced only small specimens unfavorable for study, while in other 
and good nutrient media large and favorable specimens for study. 
were obtained. Such an investigation for suitable media is, as 
these experiments and the work of Klebs have shown, an extensive 
and valuable study in itself, but space does not allow a further 
consideration of it here. 
In inoculating the various culture media above described a 
large steam-tight chest in Pfeffer’s laboratory was used. The 
glass sides of this box were first carefully washed, and then live 
steam was rapidly passed into it from a steam boiler for one hour 
or so until well sterilized. All inoculations of the culture media 
with the desired spores could then be made within the glass bas 
by proper care without the cultures becoming contaminated with 
bacteria or other forms. Unless this precaution was obse 
bacteria and other forms would frequently gain access t0 the 
cultures and interfere with the success of the experiment, OF if 
it were continued for some time, frequently outgrow and destroy 
the forms under experimentation. 
PROTOPLASMIC STREAMING IN MUCOR STOLONIFER AND M. MUCEDO 
TRANSPIRATION 
ade in order 
In these investigations a study was next m 
n saturated alr 
ascertain how the protoplasm would behave whe 
and the air of the room were alternated with each other. Accores 
ingly the spores of Mucor Mucedo were placed in a hanging drop 
culture consisting of 5 per cent pure cane sugar and arrange 
the glass ring cell as described above. The same was done 1n : 
case of spores of Phycomyces nitens and also those of Mucor stolon 
