Ce ee a a 
ANDREWS: PROTOPLASMIC STREAMING IN MuCoR 475 
TABLE III 
Trials Streamed to the Still Streamed from the moist 
dry hydrogen . hydrogen 
I 2 minutes fast I minute fast 
2 3 minute fast 5 seconds slow 
3 23 seconds slow 3 seconds slow 
4 I minute very fast Io seconds slow 
5 5 seconds still, 
starts with jerk 
6 5 seconds very fast 2 minutes very slow 
7 3 seconds still, 
starts with jerk 
8 20 seconds fast 14 seconds slow 
9 I2 seconds fast 9 seconds slow 
19 5 seconds fast 3 seconds slow 
Totals | 275 seconds 224 seconds 
eo 
It will be seen from the above figures that the time of flow in 
either direction varied greatly. The greatest amount of time, as 
well as the greatest velocity of streaming, was to the dry hydrogen. 
Inonly the first trial with moist hydrogen did the streaming appear 
fast. The totals of time above given and also the velocities 
observed are much less, as a rule, than where dry and moist air 
Were used. The specimens used in the above experiment were 
replaced in fresh air and put away for 24 hours, at the end of which 
time they were living and streamed as actively as before. The air 
used in the experiment just mentioned was made perfectly dry 
by passing it over pure glycerin (FIG. 1). Other experiments, 
however, were performed in which weaker solutions of glycerin 
Were used. It is sufficient to mention here one instance in which 
3° per cent glycerin was used. The streaming to the dry hydrogen 
i this case was much slower, as expected, and continued for 7 
minutes, which is a much longer time than the total of the same 
umber of trials as in the previous experiment. 
As has been shown above, transpiration may be practically 
Suppressed and streaming stopped or prevented by a saturated 
‘rin 20 minutes. When saturated hydrogen is passed through 
under Precisely the same conditions, the streaming stops in less 
"me than in saturated air alone. This was shown by causing 
“tturated air to be drawn over the filaments of Mucor stolonifer 
and M, Mucedo, which stopped streaming in 45 minutes by pre- 
Venting transpiration. When, however, saturated pure hydrogen 
