476 ANDREWS: PROTOPLASMIC STREAMING IN Mucor 
was drawn over the specimens the streaming of the protoplasm 
stopped in 20 minutes. This is the average time of many experi- 
ments of this kind with both saturated air and saturated hydrogen. 
As there was a difference shown by these separately, it was thought 
that a difference could be proved when they were used successively 
onthe same specimen. Accordingly, pure saturated hydrogen was 
used again on a fresh specimen and the streaming stopped in about 
20 minutes as before. Just as soon as streaming ceased, saturated 
air was drawn through and immediately a slow streaming of the 
protoplasm began and continued for 8 minutes. This and other 
experiments indicate that when the streaming of the protoplasm is 
controlled or influenced by transpiration this may be partly caused, 
as in this experiment, by physiological action. This agrees with 
Schréter’s opinion concerning transpiration in these fungi. When 
spores were sown in small drops of the solution of the different liquid 
nutrient media and a rapid growth occurred so that numerous 
long and rapidly transpiring filaments projected into only moder- 
ately dry air, the streaming of the protoplasm produced by transpl- 
ration continued often for several hours. This finally resulted, 
as Schréter states, in a concentration of the various liquid nutrient 
media to an extent that streaming to the transpiring or formerly 
transpiring tips finally ceased. If the small drops of liquid 
nutrient media be again diluted, streaming in the former direction 
will occur; and this experiment may be repeated a good many 
times with the same specimen, and always with the same result 
if due care is observed. 
. TR . 4 at 
In these experiments on transpiration the air or hydrogen th 
‘ . re 
was drawn over the specimens was warmed (in R, FIG. 1) ee 
coming in contact with the plants under investigation. ser 
periment, sul 
cases when hydrogen was employed in the ex Suit 
r 
bacteria were used in order to ascertain that no oxygen was P 
to interfere with the results. 
A series of experiments was next tried to 
transpiration of different strengths of glycerin, ; 
bination with other reagents. The glycerin mixtures did not in a 
case come in direct contact with the nutrient media in which 
fungi experimented with grew and also did not touch etsy The 
fungal filament when these projected from the culture media. 
show the effect 
also glycerin in com- 
