ANDREWS: PROTOPLASMIC STREAMING IN Mucor 477 
spores of the fungi were grown in hanging drop cultures over the 
glycerin solutions in the ordinary glass ring gas chambers. When 
good growing and streaming specimens were obtained they were 
placed, in the first of these experiments, over a 50 per cent solution 
of glycerin for 15 hours. This was much longer than it had been 
intended to allow the experiment to continue, and at the end of 
that time, as was expected, the specimens were all killed by being 
completely dried up and collapsed beyond easy recognition. This 
takes place in the filaments of the fungi here studied in a much 
shorter time, as mentioned, than 15 hours; for when the experi- 
ment was repeated, with 50 per cent glycerin, it was found that 
the fungi were desiccated to a point beyond vital recovery in 47 
minutes. 
In a third experiment of this strength of glycerin it was found 
that when a specimen of each of Mucor stolonifer and M. Mucedo, 
which were growing in a saturated air, were changed to a cell over 
a 50 per cent solution of glycerin, all streaming of the protoplasm 
stopped in 15 minutes. When the specimen was first placed over 
the glycerin solution the streaming, which to begin with was absent, 
began almost instantly and continued for a few minutes with great 
speed to the exposed portions of the filaments from which the 
water was being rapidly removed by vigorous transpiration. As 
the air became drier the streaming which at first was so rapid 
became in a short time slower and slower, as the water was re- 
moved from the filaments, and in a little longer time, as stated, 
stopped in 15 minutes. When the specimen was then changed 
back over a cell containing pure water or one through which 
Saturated air was passed, the streaming recommenced in 5 minutes 
onthe average. This recovery of the st ing under these restored 
Conditions was at first slow but in a few minutes increased in velocity 
from the tips or exposed portions which had formerly transpired 
rapidly. The increase in velocity of streaming continued till the 
filaments were again about turgid or equilibrium was established, 
When it finally ceased, as it did when they were in the saturated 
air before being placed over the glycerin. This experiment can be 
fepeated many times with the same active specimen if care is taken 
Rot to allow desiccation to progress too far. When the transpira- 
tion has been excessive for some time, the filaments are often 
