484 ANDREWS: PROTOPLASMIC STREAMING IN MuUCOR 
effect. For example, when the specimens were placed over a 20 
per cent solution of glycerin plus a 1 per cent of ethyl ether, stream- 
ing did not recommence as in the preceding experiments. When 
a new and actively streaming specimen was placed over the mix- 
ture, streaming ceased in 24 minutes. The protoplasm streamed 
Fic. 5. Apparatus for drawing a solution into the gas chamber. 
fast at first over this solution but became gradually slower till it 
stopped. The specimens were not killed, for when they were 
placed over water or saturated air drawn through the cell, streaming 
began again. The movement was at first to the base then to the 
tip of the filaments again when the air was only slightly dried. 
The time required for a recommencement of streaming under these 
conditions varied according to the state of desiccation; but on the 
average, where streaming had just ceased, it began again in moist 
air in about 3 minutes. The movement was at first from the tip, 
where absorption was occurring, and then to the tip again when 
the air of the room, which was slightly drier than that of the cell, 
was admitted. 
EFFECT OF OSMOSIS 
For the study of the effect of osmosis on protopla 
ing in fungal filaments the spores of Mucor Mucedo and 
were grown in water at a constant temperature on a g 
The cover glass was supported by bits of a cover glass 
to rest on the spores or filaments and to admit a capillary tube: 
smic stream- 
M. stolonifer 
lass slide. 
so as not 
