ANDREWS: PROTOPLASMIC STREAMING IN Mucor 489 
It has been shown that chloroform may stop streaming if used 
in a strong solution, but if used in a very dilute solution it may 
accelerate streaming or even cause it to begin.* The same is true 
of ether as regards the fungi used for this investigation. The solu- 
tion of ether used to cause streaming to begin must be very dilute 
and the protoplasm be in a condition to stream. I have not 
experimented with chloroform on the fungi mentioned in this 
paper. 
Specimens of Mucor Mucedo and M. stolonifer were grown in 
10 per cent gelatin, and then a solution of % per cent ethyl ether 
was added. The streaming ceased in a few seconds after the 
ether was applied but began again slowly in 15 minutes. This 
Same experiment was tried on other specimens of these same fungi 
with the same results. A quantity of the 1 per cent ethyl ether 
solution was also placed in the bottom of the glass cell below the 
specimens. As soon as it was found that streaming ceased for 
some time after the direct application of 14 per cent ether, a 4 
per cent solution of cane sugar was added immediately to the 
specimens whose streaming had just ceased due to the ether. 
Streaming began again in three seconds, and all movement was 
to the parts of the filaments with which the sugar solution came 
incontact. When the sugar solution was washed away and water 
added, the streaming began but in the reverse direction. 
Another series of the same fungi were grown as in the preceding 
€xperiment, but this time a ly per cent solution of ethyl ether was 
added directly to the specimens growing in 10 per cent gelatin. 
The protoplasm stopped streaming almost as soon as the ether was 
applied. The specimens were not killed by the addition of ether 
of the above strength, for when the ether was replaced with water 
the Streaming recommenced and after a time regained its normal 
Velocity. If a 4 per cent solution of cane sugar was applied 
immediately to the specimens that had just ceased to stream 
due to the 14 per cent ether, streaming began in from 10 to 15 
*Hauptfleisch, P. Untersuchungen iiber die Strémung des Protoplasmas in 
behduteten Zellen, Jahrb. Wiss, Bot. 24: 220. 1892; Ewart, A. J. Protoplasmic 
og ing in plants 87. 1902; Pfeffer, W. Plant Physiology 3: 319. 1905; Josing, 
: eaib. Wiss. Bot. 36: 210. ; 
: Ewart, loc. cit. 86; Josing, loc. cit. 210. 
