SCHREINER & SKINNER: EFFECT OF GUANIDIN ON PLANTS 547 
TABLE III 
EFFECT OF GUANIDIN IN CULTURE SOLUTIONS CONTAINING NITROGEN AS NITRATE 
ND AS ASPARAGIN 
Culture solution Green weight, grams 
oc elgg 
be soil erewcad Se ale uk 428 4 Cr Ee MOI ECON Ce Gs ot Pp eee au eee Sa 4.0 
oo. 
CaHa(POs,)2 
maw Os 4 25 parts per million guanidin ©... 65 5 6 0 es 5 ees 2.5 
K2SO4 
se 
Rs oo 8 5 Gas ow a ew nae og al Pee a tw ai eee ew ee 3.5 
K:S0. 
“epi sje 
paragin + 25 parts per million guanidin............... cece eee eeee 3-3 
ooo. 
Creatinin was also tried in this same manner. Creatinin was 
identified as a soil constituent in this laboratory * and also found 
to be a constituent in a number of plants.t Its effect on plants 
has been shown to be beneficial, its action being to replace nitrate 
in producing plant growth.t The results obtained by using 
creatinin as the source of nitrogen instead of sodium nitrate in 
conjunction with the guanidin are given in TABLE IV. 
TABLE IV 
EFFECT OF GUANIDIN IN CULTURES CONTAINING NITROGEN AS NITRATE AND AS 
Culture solution Green weight, grams 
ree 
bias ad C8 Viele CUES 2h ee PARES ESE ECs Eee ene RT eS 3-4 
CaH«(PO.)2 
— + 25 parts per million guanidin .........---0++eseeeee eee eee 2.4 
{Gres Dose pa 
Dre Bini i Sere Nr ea all es aie eee a ae en a 3.0 
oes 
inin + 25 parts per million guanidin.......----++-+++sreereeree 3.0 
F< Gicecy, Shorey, E. C. The isolation of creatinin from soils. Jour. Amer. Chem. Soc. 
34:99. 1912. 
t+ Sullivan, M. X. The origin of creatinin in soils. Jour. Amer. Chem. Soc. 
33: 2035. 1911. 
t Skinner, J. J. The beneficial effect of creatinin and creatin on growth. Bot. 
- 54: 152-163. f. z. 16 Au 1912 
