72 INLAND FISHERIES. 



trace of any new arms. Of specimen 2 only one piece (A) was 

 alive. From the stump of the old arm a very small new arm 

 appeared — no trace of any other. The two pieces A and B of 

 specimen 3 were alive and healthy. In A two very small arms 

 were visible (one could be seen only with the help of a hand lens) 

 near together, and on one side of the cut surface ; on the other 

 side there was a trace of another arm, indicated by an eye spot. 

 B had healed up, but showed no trace whatever of regenerating- 

 arms. Of the fourth specimen piece A was found alive with two 

 very minute regenerating arms. These specimens had very little 

 food, and it is hardly necessary to remark that they grew very 

 little or not at all. 



On September 5, 1898, another experiment, similar to the last 

 one, was commenced at Kickemuit River. Eight specimens were 

 taken from those reared in the car, and cut in two in the manner 

 shown in Fig. 29, leaving two arms and the madreporic plate on 

 one piece, and three arms and part of the plate on the other. The 

 pieces of the latter sort died in a short time, and the folloAnng 

 tabulated data refer to the pieces having two arms and the plate 

 (" eye"). At the beginning of the experiment the sjiecimens 

 measured in millimeters, 23, 21, 21, 20, 19, 18, 18, 18. 



September 2Gth, {Three weeka after the operation.) 



A 20 mm., bore trace of two new arms. 



B 18 mm., two new arms (preserved) unhealthy. 



C 18 mm., one new- arm, 2 mm. 



D Crushed. 



E 20 mm., healthy — no trace of another arm. 



F Overlooked. See next, October 12. 



October 12th. 

 A (?) 23 mm., two very small arms. 

 C 20 " two arms, one smaller than the other. 



E (?) 22 " no trace of regeneration. 

 F 21 " one arm. 



(all healthy.) 



