330 CALIFORNIA ACADEMY OF SCIENCES. [Proc. 3D Ser. 



I. Strong Flemming : 



1 per cent, chromic acid 15 pts. 



2 per cent, osmic acid 4 pts. 

 Glacial acetic acid i pt. 



2-6. No. I diluted with 1-5 volumes of water. 

 7. I per cent, chromic acid 15 pts. 



2 per cent, osmic acid 4 pts. 



I per cent, glacial acetic acid i pt. 

 8-12. No. 7 diluted with 1-5 volumes of water. 

 13. I per cent, chromic acid 15 pts. 

 2 per cent, osmic acid 4 pts. 

 14-18. No. 13 diluted with 1-5 volumes of water. 



19. /^ per cent, chromic acid 15 pts. 

 2 per cent, osmic acid 4 pts. 

 Glacial acetic acid i pt. 



20. No. 19 diluted with i volume of water. 



21. I per cent, chromic acid. 



22. 2 per cent, osmic acid. 



23. I per cent, osmic acid. 



24-26. I, 5 and 10 per cent, aqueous solution of bichro- 

 mate of potash. 

 27. Tellyesniczky's fluid (Arch. mikr. Anat. 52:247, 

 1898) : 



Bichromate of potash 3 gm. 

 Glacial acetic acid 5 cc. 

 Distilled water 100 cc. 



28-32. No. 27 diluted with 1-5 volumes of water. 



33. Miiller's solution: 



Bichromate of potash 2-2^ pts. 



Sulphate of soda i pt. 



Distilled water 100 pts. 



34. Zenker's mixture : 



Corrosive sublimate 5 per cent. 

 Glacial acetic acid 5 per cent. 

 Miiller's solution 90 per cent. 



35. ^Saturated aqueous solution of bromine i pt. 



iThe material was immersed for two hours in this solution, and then removed to 

 Flemraing's strong mixture, where it remained eight hours. 



