Box. -Vol. II.] HUS— CASSIA TOMENTOSA L. 335 



be seen connected with the chromosomes. The nucleus 

 is more or less completely surrounded by a zone of smooth 

 parallel fibers which stain a dark blue. Next these lies 

 the granular zone, occupying about half the remaining 

 cell-space, and composed of a reticulum of small, more or 

 less isodiametric meshes, between which (especially at the 

 intersection of the threads) a great number of larger and 

 smaller granules may be seen. Surrounding this zone 

 (which stains brown-violet) is a dark blue line apparently 

 composed of fibers upon which granules, large and small, are 

 situated. These fibers are not continuous, and the number 

 of granules upon them is not the same in all places, so the 

 ring which they form is an irregular one. It stains a deep 

 blue, very effectually marking the boundary between the 

 brown-violet granular zone and the outer gray-yellow cyto- 

 plasm, which is composed of fibers forming large irregular 

 meshes. Upon and between these fibers are larger or smaller 

 masses of granules. The granular zone does not present 

 an absolutely uniform appearance; in places fibers upon 

 which granules have accumulated stand out clearer than 

 others. Usually they run parallel to, or at a small angle 

 with the nuclear wall. At first only one or two are seen, 

 but they rapidly increase in number. Soon those nearest the 

 nuclear wall seem to establish connection with some of the 

 fibers of the felt-like zone (fig. 5) ; gradually other, deeply- 

 staining fibers are added, all running at a greater or lesser 

 angle to the nuclear wall, and finally arranging themselves 

 in conical groups with their bases resting on the fibrous 

 zone. Not only one cone like this may be observed, but 

 several (fig. 6). During this time the number of prominent 

 fibers in the granular zone continues to increase. Fre- 

 quently a space can be observed, at least in places, between 

 the nuclear wall and the felt-like zone. 



At about this stage the nucleolus begins to disappear; in 

 the preceding one, however, the nucleolus was still present, 

 and the chromatin was in the form of curved, rod-shaped 

 chromosomes, or else in the form of rings, formerly con- 

 sidered so typical of the Liliaceas. 



