447 
and semi-opaque centre, moving freely on each other, and pre- 
senting the Brownian movement. 
b. Bodies of larger size, strongly refractive, varying in dia- 
meter, and evidently oil-globules. 
c. Vesicles of various sizes, round, oval, and.elliptical, some 
presenting coarsely granular contents, others extremely fine, 
almost homogeneous, contents, their borders uniformly clear, 
dark, but fine, and readily seen; these vesicles were, some as 
large as ordinary cells, others as small as nuclei. In none could 
I detect anything like a nucleus or nucleolus. 
2. A portion of the germinal membrane was found to pre- 
sent— 
a. Numerous granules, butin much less quantity than in the 
body of the yolk ; they were also clearer and more transparent. 
b. Vesicles of various sizes, with fine pale contents, and clear 
but well-defined border, occasional oil-globules, granular cor- 
puscles, with large, dark, spherical contents, and well-defined 
dark border. These corpuscles varied much in size; aggre- 
gated masses of granules existed here and there, both of the 
light and dark variety of granules, and with corpuscles im- 
bedded in them. 
In no case did I see a distinctly nucleated cell in this pre- 
paration, many vesicles contained a central mass of spherules, 
but no distinct nucleus. The superposition of granules or ve- 
sicles is very likely to be mistaken for nuclei or nucleoli; occa- 
sionally a motion in the fluid disturbs the granules, and they 
may then be seen to float freely away. I have often observed this. 
3. In a preparation from near the germinal centre, after 
about twenty-four hours’ incubation, the vesicles were much 
larger, more clearly seen, and very finely granular for the 
greater part ; a few presented central spherules, with or with- 
out granules, but I am doubtful, for the reasons just assigned, 
whether they are to be regarded as nuclei ; a few coarsely gra- 
nular vesicles were to be seen; also, isolated and aggregated 
granules. ’ 
