449 
blood ceased to flow, I found not only granules, but granular 
corpuscles, hyaline vesicles, and granular masses. In another 
instance, one hour after the receipt of a wound in the hand, I 
examined the exudation, and found a well-marked granular 
base of considerable extent, abundance of granules, and a few 
examples of nucleated cells. 
7. The observations of Robin and Handfield Jones, on the 
development of fat, likewise prove a mode of growth not re- 
concilable with the nucleo-cellular doctrine. 
8. Another class of proofs may be deduced from the re- 
sults of experimental or artificial Histogenesis, which go to 
prove the direct formation of tissues, without the intervention 
of cells. | 
Thus, in the well-known experiment of Ascherson, the 
contact of oil and albumen, two homogeneous fluids, gives rise 
to the formation of granules, granular base or stroma, vesicles, 
and simple membrane (hyaline membrane). 
The experiments of Panum show the possibility of artifi- 
cially forming granules, vesicles, and granular corpuscles. 
The results obtained by Melsens, and fully confirmed by 
microscopic examination of his ‘tissu cellulaire artificiel” both 
by M. Gluge and myself, give us instances of the direct for- 
mation of at least three elements of organic bodies, indepen- 
dently of cells, viz., granules and granular base, fibres, and 
corpuscles.* Similar results have been obtained by Parkes. 
I am able to furnish another and valuable class of proofs 
from the results of my researches in Histolysis, which show, 
as will be fully detailed further on, that structures can originate 
under conditions when we cannot suppose any vital organic 
influence to be present, but when such forces as attraction, 
cohesion, fusion, endosmose and exosmose, and the mutual 
* For an account of Melsens’ experiments, see Dublin Quarterly Journal 
of Medical Science, February, 1852. For Panum’s experiments, see Lyons’ 
Annals of Micrology, British and Foreign Medico-Chirurgical Review, April, 
1853. 
