New York Agricultural Experiment Station. 173 



temperature and then treated with benzoyl chloride in dilute 

 alkaline solution according to the Schotten-Baumann method.^ 

 This method has been employed by Lang-stein-* in the separation 

 of oxyphenylethylamine formed by an intense peptic digestion 

 of egg-albumin. An abundant precipitate separated, which was 

 filtered and washed with cold water. It was then dissolved in 

 hot alcohol and evaporated to small bulk. On standing, an 

 abundant crop of crystals separated, which were filtered, washed 

 with ether and dried over sulphuric acid /;/ vacuo. 



This product had a melting-point of 169° C. (uncorrected), 

 agreeing exactly with the oxyphenylethylamine obtained by 

 Langstein. The following results were obtained by determining 

 the nitrogen by the Kjeldahl method and the carbon and hydro- 

 gen by combustion. 

 Calculated for benzoylderivative of oxyphenylethylamine. Found. 



Cs H, NO (Ce H, CO), 



c 76.50 76.19 



H 5.54 544 



N 4.06 4.10 



This product was undoubtedly oxyphenylethylamine formed, 

 as we shall point out later, from tyrosine with the accompani- 

 ment of carbon dioxide as a by-product. 



Heron bases. — The remainder of the phosphotungstic acid 

 precipitate, obtained in the manner previously described, was 

 decomposed by barium oxide and the excess of barium 

 hydroxide was removed by careful addition of sulphuric acid. 

 The resulting filtrate was worked for the hexon bases accord- 

 ing to the Kossel-Kutscher^ method. 



(i) Argininc. — After separating histidine from the solution, 

 which should contain only argini'ne and histidine, a determina- 

 tion was made of the nitrogen in this solution containing only 

 arginine. The amount of arginine, thus determined, equivalent 

 to the nitrogen found, was only 0.364 gram, an amount too 

 small to obtain in the form of crvstals. 



^Ber. d. chem. Ges., 17: 2545 (1884) and 19: 3218 (iJ 

 ^Beit. z. Chem. Physiol, und Pathol., 2: 229 (1902). 

 ^ Ztschr. physiol. Chem., 31 : 165 (1900). 



