174 Report of the Chemical Department of the 



(2) Histidinc. — This substance was separated as the di-chloride, 

 of whicli we obtained 0.850 gram. An analysis gave the follow- 

 ing results: 



Calculated for histidine hydrochloride. Found. 



(Ce H« N3O2 2HCI) 

 N 18.42 18.15 



CI 3 1. 1 1 30.98 



(3) Lysine. — We separated about 2 grams of lysine in the form 

 of picrate, which gave the following results on analysis: 



Calculated for lysine picrate. Found. 



(CoH,, N,0, C.,H3 N,0,) 



N 18.66 18.80 



C 38.40 38.44 



H 4-53 4-56 



(4) Guanidinc. — The mother-liquor from the lysine precipitate 

 was extracted by a mixture of alcohol and ether and then treated 

 with gold chloride in very dilute hydrochloric acid solution, fol- 

 lowing the method of Winterstein and Thony.*^ On standing, a 

 crystalline substance soon separated from the solution, behaving 

 like a guanidine gold salt, yielding about 0.300 gram, which on 

 analysis gave the following results: 



Calculated for guanidine gold chloride. Found. 

 (CH,N3 HCl AuCL) 

 Au 49.79 49.63 



So small an amount of this substance was obtained that we 

 were unable to make other determinations to establish its 

 identity with greater certainty, but it is highly probable that the 

 substance is guanidine. 



(5) Putrcscinc. — We expected to separate the other cleavage 

 product of arginine, putrescine. The lysine solution had a 

 strong odor of putrescine and it was unquestionably present but 

 we failed in our efforts to isolate this base. It appears probable 

 that the cleavage of arginine had only progressed as far as the 

 formation of guanidine and ornithine, and that the latter com- 

 pound had been decomposed only to a small extent, forming 



^Ztschr. physi:yl. Chcm., 36: 28 (1902). 



