New York Agricultural Experiment Station. 203 



ing to Bliss and Novy,^ pepsin is not affected by a i per 

 ct. solution of formaldehyde nor rennet by a 4 per ct. solu- 

 tion. In one set of fiasks, we added to each 0.06 gram of the 

 sterilized pepsin, and in each of the other set of flasks 0.5 cc. of 

 the sterilized rennet-extract. Duplicates were used in all cases. 

 These were examined bacteriologically and chemically at intervals 

 for 3 months. The formalin was very effective in destroying 

 bacterial forms. In some cases a few molds were found, but 

 not in sufficient number to affect the work. The nitrogen in the 

 material was 4.35 per ct. 



Table V. — Showing Effect of Rennet-Enzyme and Commercial Pepsin 

 ON Paracasein Dilactate. 



From the data contained in Table V, we can see that the 

 results of our work indicate that: 



(i) Both pepsin and rennet-enzyme exerted a marked proteo- 

 lytic effect upon the paracasein dilactate, digesting about one- 

 third of it in 2 weeks and considerably over one-half in 3 months. 

 While the rennet-enzyme appears somewhat more active in form- 

 ing water-soluble nitrogen compounds, the actual- difference is 

 small. 



(2) Both enzymes formed amides in small quantities, but 

 neither produced any ammonia. 



(3) If we compare the results in Table V with those in Table 

 III, we find that at the end of i and 3 months, more proteolysis 

 "Occurred in this experiment than in the presence of chloroform. 

 This is true of both enzymes. This suggests that the chloroform 



^ Jour. Experimental Med., 4: No. i (1899) 



