New York Agricultural Experiment Station. 287 



days the samples were bottled and analysis made by the hydro- 

 chloric acid method. Below we give the data and, for comparison, 

 results obtained on the imground seeds. We also present data 

 secured on the two weeks old germinated seeds by the Iwanow 

 method. 50 cc. of ordinary molybdate were used to 200 cc. of 

 extract. Iwanow does not state the exact quantity of molybdic 

 solution which he used, so we are unable to repeat his method 

 exactly. 



Table VI. — Phosphorus in Germinated and Ungerminated Grains. 



We see from the above figures that during the germination of 

 these seeds in the dark, there is a marked increase in soluble 

 organic phosphorus combinations, but that according to our 

 method of estimation the cleavage does not extend to the forma- 

 tion of inorganic phosphates. The increase of inorganic phos- 

 phorus accompanying the increase of total soluble organic 

 phosphorus, is explained by the slight cleavage action of our 

 reagents. 



The Iwanow (^^ method in our hands gives results in perfect 

 agreement with his previously reported results— that is, that dur- 

 ing germination in the dark there is a marked formation of 

 inorganic phosphorus. 



The discrepancy in the two results appears to be due to differ- 

 ences of method. Araki^-^ working on the action of certain 



(1) Reference cited above. 



(2) Zeit.f. Physiol. Ckem., 38: 84, (1903). 



