a 
New York AGRICULTURAL HXPERIMENT STATION. 105 
TABLE X.—SHOWING DIGESTION oF CASEIN MONOLACTATE AND 
CaseEIN Dintacrare By PrEepsIn Wirsuout Aci. 
PERCENTAGE OF NITROGEN IN MATERIAL, Ex- 
PRESSED IN FoRM OF WATER-SOLUBLE NITRO- 
GEN. 
MATERIAL. Nitrogen Total : 
in nitrogen In 4 In 8 In 24 In 48 In 72 
material. in each hours hours. hours. hours. hours. 
bottle. 
: Per ct. Gram. Perict. | Her et, | Beret. |) Per cts |) Pericts 
Casein dilactate ...., 2.34 0.830 8.56 11.81 21.69 22.65 23.00 
Casein monolactate. . 4.15 0.830 14.34 Lora 23.61 26.63 29.64 
The casein monolactate digested in the absence of free acid 
somewhat more rapidly than the dilactate. This result we did 
not anticipate, since it was expected that the compound contain- 
ing the higher amount of acid would be acted upon more quickly 
by pepsin. It may be suggested, in explanation of this behavior, 
that the acid in the dilactate appears to be in more stable combi- 
nation than in the monolactate. 
(8) Comparison of the digestibility of paracasein monolactate 
and paracasein dilactate with and without acid.—The paracasein 
dilactate was prepared by allowing 20 pounds of milk and half a 
pound of sour-milk starter to stand eight hours at room tempera- 
ture and then adding 0.5 cc. of rennet-extract, after which it was 
allowed to remain until quite acid. The monolactate was ob- 
tained in the form of new cheddar cheese, in which 66.21 per ct. 
of the nitrogen was in the form of monolactate. These materials 
were prepared for digestion as described under (1) preceding, 
except that we used 0.300 gram of pepsin, and 1 ce. of chloroform, 
and in experiments 3 and 4 we used in addition 0.4 per ct. of 
hydrochloric acid. 
TABLE XI.—SHOWING DIGESTION OF PARACASEIN MONOLACTATE AND 
Divactate Witsh AND WirHouTt AcID. 
| 
| Prercenrace or NuirroGeN IN Materiat, 
| EXPRESSED IN ForM OF WATER-SOLUBLE 
Hydro- Total NITROGEN. 
chloric nitrogen 
MATERIAL. acid ineach | 
used in bottle. | In4 | In8 In 24 | In48 | In72 
digestion. | | hours. hours. hours. hours. hours. 
Per ct. Gram. | Perct. | Perct. | Perct. | Per ct. | Per ct. 
1. Paracasein mono-! 
lactates..56 hohe h 0. 0.710 23 .66 27.46 | 49.72 51.84 | (oy4 ailal 
2. Paracasein  dilac- | 
GALS R years se suas 0 OL TUG! ie Wasa SAS oT) Nee. Bz 31.83 oo 0e, 
3. Paracasein mono- | 
ENG ENTS Ben cae Gaaeee 0.4 0.710 | 48.31 | 56.63 AG. Gi \> DLeoo BY f ash) 
4, Paracasein dilac- 
ULES to) lees mate. se 0.4 0.710 67.75 Tanoo: 70.43 67.89 73.39 
