New York AGRICULTURAL EXPERIMENT STATION. 115 
acid. Data illustrating this point will be found in the following 
table : 
TasLe J.—So_usLe PuHospHorus IN Oats, Mair Sprouts AND 
WuHeat Bran. 
Seca Solvent. ouecee mis et total 
Per ct. Per ct. Per ct. 
Griges bp tc lie 0.355 Hydrochl Fe OAen 20.0 
Malt sprouts...... 0.677 } ue oer 02S oan 
Wheat bran....... 1.22 ; Mosc nchlovie oe ee 
It will be seen that wheat bran carries a much larger per- 
centage of phosphorus than any of the others and that 86.5 per 
ct. is soluble in water. 
It was at first supposed that the soluble phosphorus was in 
combination as nucleins or salts of nucleic acid, but determina- 
tions of the amount of soluble nitrogen showed that only about 
33 per ct. of the phosphorus could be accounted for in this way. 
We were therefore led to the conclusion that by far the greater 
part of the soluble phosphorus was linked up in some other 
organic combination, since Hart and Andrews’ have shown that 
the amount of soluble inorganic phosphorus is exceedingly 
small. 
INVESTIGATION OF PHOSPHORUS COMPOUND. 
ATTEMPT TO ISOLATE. 
Two pounds of wheat bran was extracted with six liters of 0.2 
per ct. hydrochloric acid for several hours with frequent stir- 
ring. The extract was strained through cheese cloth and finally 
filtered through paper. 
The filtrate was treated with a large volume of 95 per ct. 
alcohol which threw down a voluminous, flocculent precipitate. 
The precipitate was allowed to settle to the bottom, the superna- 
tant liquid syphoned off and the precipitate washed with alcohol 
several times by decantation. It was then dissolved in a small 
volume of 0.2 per ct. hydrochloric acid, filtered from an insolu- 
_ble residue, reprecipitated by alcohol and washed as before by 
decantation. 
_ ? Bulletin 238, N. Y. Agr. Expt. Station. 
