New York AGRICULTURAL EXPERIMENT STATION. ai hy 
larger part of the precipitate went into solution again on cooling 
leaving the coagulated proteid in suspension, which was sep- 
arated by filtration. The filirate was again heated and the result- 
ing coagulum collected on a hot water funnel. 
Schulze and Winterstein*® continued the investigation started 
by Palladin and obtained a compound carrying 9.65 per ct. C 
2.83 per ct. H and 16.13 per ct. P. They thought the sub- 
stance might be identical with the chief constituent of the globoids 
found enclosed in the protein kernel of many plant seeds. 
Pfeffer in conjunction with Brandau found that such globoids 
consist of a calcium-magnesium salt of an organic body coupled 
with phosphoric acid. What the nature of this organic com- 
bination was they were unable to say. 
Winterstein® isolated a compound from the seeds of black 
mustard by extracting with dilute acetic acid. The extract was 
freed from proteid by boiling and filtering when cold. The fil- 
trate was made slightly alkaline with ammonium hydrate and 
boiled, the resulting precipitate collected on a hot water funnel 
and washed with hot water until the wash water reacted neutral. 
The substance was purified by dissolving in dilute acetic acid and 
reprecipitating with ammonium hydrate. On removing the 
calcium with oxalic acid, a product was obtained carrying 18.44 
per ct. P and 7.88 per ct. Mg. By heating the magnesium salt 
in a closed tube with concentrated hydrochloric acid for 50 hours, 
at a temperature of 180°—140° he obtained inosite as a cleavage 
product. 
The compound obtained by us from bran by the alcohol method, 
though still impure, is undoubtedly identical with the one 
described by these investigators. 
Posternak® isolated the compound from the seeds of red fir, 
pumpkin, peas, beans, white and yellow lupine and also from the 
3Ueber einen phosphorhaltigen Bestandtheil der Plfanzensamen. Zeit. 7. 
physiol. Chem., 22: 90. 
4W. Pfeffer: Untersuchung ueber die Proteinkoerner und die Bedeutung des 
Asparagins beim Keimen der Samen. Jahrbiicher fiir wissenschajtliche Botantk., 
8: 147. (1872). 
’ Ueber einen phosphorhaltigen Pflanzenbestandtheil, welcher by der Spaltung 
Inosit liefert. Ber. d. deut. chem. Ges., 30: 2299. 
® Revue Generale de Botanique. 12: 5 and 65, 1900. 
Comptes Rendus, 137: No. 38, (20 Juillet. 1903). 
No. 5, (3 Aout 1903). 
No. 8, (24 Aout 1903). 
