New York AGRICULTURAL EXPERIMENT STATION. 121) 
methylorange with deci-normal barium hydrate required 5.1 c. e. 
of 0.035 gm. barium. 0.0648 em. of the acid should require 0.0343 
gm. barium to produce the acid salt (C. H. P. O. Ba.). 
QUANTITATIVE ESTIMATION OF THE ACID IN WHEAT BRAN. 
We extracted 10 gms. of bran of 500 ¢. c. with 0.2 per ct. hydro- 
chloriec acid, made 100 c.c. alkaline with sodium hydrate, pre- 
cipitated with barium chloride filtered and washed free from 
alkali. 
The phosphorus was determined directly in this precipitate: 
Peer PNOSpHORIS,, Per CCNt.<. no fo coe tla os ck te case evesawnee 1.25 
Ehospkorus in barium precipitate, per cent. .......5.....se2.--s. 0.92 
Per cent. of. phosphorus barium precipitate to total phosphorus, 
ETACCTU ree Perera ahs, ctterars statis nya ee Ainisteec aCe crcvate ta Mlanercuert nate cic 58.1 
Practically all of the phosphorus soluble in 0.2 per ct. hydro- 
chlorie acid is in this form as is shown below: 
Hcl soluble) phosphorus, | per cents... 2).Js0 sido. we). veh Jeoeaes bc 0.94 
Phosphorus in barium precipitate, per cent.............c00eeeeee 0.92 
- Per cent. of phosphorus barium precipitate to soluble phosphorus, 
DEP GETS cod cs RNa Orotete noe conte Ore ee a ace ate ee ea ae 97.9 
