226 
poured off, the propionie aci: regenerated, by salting it out by 
means of CaCl],, and rectifying the upper layer. The recovered acid 
(about 800 em?) together with 200 em“ of fresh acid were treated 
again with 200 em? of blood and the mother liquor used once 
more after being purified. 
In all we obtained: 
1 “ preparation: 0.515 gr. haemin 
9a or 
2 ; 0.535 , 5 
BI = 0.750 , 5 
The haemin obtained in this manner was quite pure. An exa- 
mination of the crystalls, which were well developed, through the 
microscope proved the absence of any amorphous impurities. The 
erystalls were large but otherwise quite identical with those of acet- 
haemin, as will be seen from the appended photographs (Plate IV). 
Fig. 1 & IT represents haemin obtained by means of propionie 
acid, III ,acethaemin“ and IV ,acethaemin“ obtained from Mör- 
ners haemin !). 
The composition corresponds exactly to the formula C;, H;,O, 
ClFe — 652, as will be shown by the following results of several 
analysis: 
1) 0.1291 gr. gave 0.2960 gr. CO, (after Messinger) 
2) 0.1430 „ 052627 0, à = 
3) 0.1254”, ,„ 04927, CO, and 0.1071’er. H,O 
by combustion and 0.0261 gr. Fe, O, (residue) 
4) 0.2110 gr. gave 16.3 cm? N (t— 16.5, p — 744 mm) 
C,, H3s 0, CIFe 
1) C— 6253), 2)—6221°,, 3)— 62.65 62.48), 
3) H—552 506 „ 
3) Fe — 849°), 8.59 , 
4) N— 8:64!) 8.60 „ 
The physical properties of haemin prepared by means of pro- 
pionie aeid and that made by using acetic acid are quite identical. 
A comparison of the spectra of their chloroformie solutions showed 
their identity. In dilute solutions of either of them three bands are 
1) Comp. this Bull. 1903 p. 795. 
