N 
=] 
( 
Phylloerytrine in chloroform Cholehaematin 
(Marehlewski) (Mac Munn & Gamgee) 
Band I: A — 642 — 640 Band I: À — 649 — (centre) 
„ IH: À — 606 — 581 „ II: A-- 613 — 585 
„ Ill: A — 577 — 557 „ Il: A — 577.5 — 561.5 
„ IV: 21—536 — 515 „ IV: A — 537 — 5215 
I thought it therefore worth while to compare phylleorytrine and 
cholehaematin by direct observation. 
The contents of several gall bladders of sheep which were fed 
exelusively on grass, were left to stand for two days in the pre- 
sence of air, a little water added and the mixture which was of 
an olive colour with a reddish hue examined through the spect- 
roscop. I notieed four absorption bands. The first is very badly 
deffined, its more refrangible edge corresponds to the wave length 
635, the position of the other edge it was impossible to ascertain 
in consequence of a more or less continuous strong absorption of 
the orange and red rays. The second band is determined by the 
following wave lengths: A — 611 — 685.5, the third À — 577 — 563, 
the fourth 2 — 534.5 — 522. 
The next measurements were carried out using a ehloroformie 
extract of the bile, which I prepared more or less according to 
Mae Munns perscription. The gall-bladder contents were acidulated 
with acetic acid, absolute alcohol added, filtered and the filtrate 
extracted with chloroform. The chloroformic extraet possessed an 
olive colour with a very strongly ponounced reddish tint. A direct 
comparison of this solution with a chloroformie solution of phyllo- 
porphyrin containing a little chloroform, taking care that the con- 
centrations correspond with each other as elosely as possible proved 
that their spectra are indeed almost identical. The positions of the 
bands correspond to the following wave lengths: 
Band I Beginning of band 2 638 Beginning of band 2 637 
A À — 604 — 582 À — 603 — 580 
mn À — 572 — 559 7202508 
IV Ber 2 — 530 — 515 
The bands caused by the cholehaematin solution are scarcely 
perceptibly shifted towards the red end of the spectrum as com- 
pared with those of phylloerythrine. Aceording to their intensities the 
