144 
a greenish yellow colour; it was evaporated again to dryness, 
dissolved in water and acidulated with hydrochlorie acid, and 
finally extracted with ether. The etheral solution appeared now 
yellowish and in its spectrum only the two most pronounced cho- 
lehaematin bands were distinguishable. The ether was again eva- 
porated and the residue dissolved in a very small quantity of 
chloroform. The colour of this solution was vellowish brown, and 
the cholehaematin spectrum very badly pronounced; visible were 
only three bands, the first in the orange was absent. The next 
portion of bile was drawn on the 8" of June, in the evening and 
examined on the 9", applying the same procedure as stated before. 
Cholehaematin proved to be still present although in very small 
quantities. On the 10" another portion of bile was drawn and exa- 
mined on the following day. The quantity of cholehaematin present 
I determined approximately colorimetrically in the following manner. 
One milligram of pure phylloerythrine was dissolved in 100 eem 
of ehloroform and the strength of the eoloration produced eompared 
with that eaused by cholehaematin, obtained from the bile. It was 
found that the latter eould not have contained more than about 
0:0005 gr. of the eolouring matter. The bile drawn on the 13” of 
June still contained some cholehaematin, but certainly less than 
0:0005 gr. The bile taken on the 15" did not contain at last any 
cholehaematin; instead another eolouring matter was observed under 
the following circumstances. The first ethereal solution, obtained 
as deseribed above gave on evaporation a green grease which dis- 
solved in chloroform with a green colour. In the spectrum no cho- 
lehaematin bands were observed, but instead of those a band in 
the red; an addition of hydrochlorie acid eaused the green colour 
to be replaced by a yellow one. and the band at the same time 
disappeared. 
On the 17* of June the animal was fed again with grass and 
the bile drawn and examined on the 20". The bile appeared in 
greater quantites than in the former feeding period with dry food. 
which eonsisted of oats; its colour was yellowish brown and it 
showed without any further treatment the cholehaematin bands. 
From the 20" of June up to the 26" the bile was colleeted and 
the phylloerythrine eontained in it determined colorimetrieally; 
its quantity amounted to about 0'008 g. 
The result of these experiments is quite clear: there cannot be 
