795 



54. MM. J. HETPEE et L. MAECHLEWSKI m. t. Studya nad barwikiem 

 krwi, I. (Studies on the blood colowring matter, 1" preliminary 

 note). (Recherches sur la matière colorante du sang). 



The au t hors studied the corelation i>f so called p- haemin <>f 

 Moerner and aeethaemin, described by Nencki and Zaleski. The 

 latter authors ascribed to aeethaemin the formula C 34 H 33 4 N 4 FeCl 

 and consider Moemers haemin to be an ethylaether of aeethaemin. 

 This conclusion has been opposed by W. Küster who believes 

 b- haemin to be a haemin sui generis. Aceording to the latest re- 

 searches by Zaleski, „aeethaemin" is a misnomer, and the substance 

 known under this name must be considered as the first coloured 

 product, produced from haemoglobin under the influence of hydro- 

 lytical agents. Under thèse conditions it was necessary to clear up 

 if possible, the real corelation of the two substances named. We 

 propose to conimunicate in this preliminary note the results we 

 hâve obtained hitherto. and we hope to be able to return to the sub- 

 ject on a further occasion. 



Based upon a séries of experiments, of winch only a few will 

 be named hère, we arrived at the conclusion that the composition 

 of Moerners haemin dépends in a considérable degree on the phy- 

 sical conditions, under which the experiments are carried out. and 

 that. in most cases, this haemin contains only very little of etho- 

 xvl groups, although it is never quite free from them. In other 

 words. we find that firstly, the view advanced by Nencki and Za- 

 leski concerning Moerners haemin eannot be applied in gênerai, 

 although we deem it quite possible that under certain conditions 

 une may obtain a produet that will contain as much as 6 - tt°/o OC, H 5 

 in form of ethoxyl groups. and secondly that Küsters contention 

 eannot be upheld. In our opinion Moerners haemin is a mixture 

 of aeethaemin with greater or smaller quantities of its ethers. a view 

 based upon the fact that it is possible to obtain aeethaemin from 

 Moerners haemin. 



Aeethaemin. 1 L. of glacial acetic acid. saturated with sodium 

 chloride, was heated to 95° and added to this 200cm 3 of blood (free 

 from fibrome); the mixture was heated up again to 95° and left 

 to cool and crvstallize. We obtained well developed crystals corre- 

 sponding exactly to the description given by Nencki and Zaleski 

 The crystals were recrystallized according to Schalfiejew's method, 

 and gave on being analyzed the following results: C: 62 - b , 5°/ (r 



