94 W. M. Smallwood 
The chromosomes which are in the polar cells pass through a 
number of states which so far as I am aware have not been pre- 
viously recorded. They are as follows: When the first polar cell 
is formed the chromosomes, as already stated, do not usually pass 
into a vesieular condition until after its complete division. These 
two polar bodies loosely attached to the cell wall frequently are 
pushed to one side during the formation of the second polar cell. 
In Fig. 15, the chromosomes are still free in the polar cell, while 
in Fig. 18, they lie enclosed in a definite nuclear vesicle and have 
lost some of their distinetive appearance and are associated with 
linin fibree. The changes connected with the chromosomes in the 
polar cells can not be correlated in time with similar changes in the 
egg chromosomes. 
Clearer evidence in regard to these changes was furnished in 
the second polar cell. This cell is often quite large (Fig. 22), so 
that its condition can be readily observed. Each chromosome is 
surrounded by a vesiele so that we may have as many chromosome 
vesicles as there are chromosomes, or all or several pass into one 
vesiecle. In Fig. 15, the egg ehromosomes are in separate vesicles 
having linin fibres present. There is not the close grouping usual 
at this stage. This is further shown in Fig. 16, where considerable 
eytoplasm intervenes between some of the vesieles. But in the se- 
cond polar eell (Fig. 15) the chromosomes all lie in one vesicle, 
showing no further evidence of entering a nuclear state, the presence 
of linin fibres and the ordinary differential conditions present being 
entirely absent here. While the conditions in Figs. 16 and 18, reveal 
but a single vesicle for the second polar cell yet there is plenty of 
evidence of linin fibres and the chromatin gives a differential reaction 
to haematoxylin in that certain of the granules take a very heavy 
stain while others react but slightly. 
The character and number of the chromosome vesieles formed 
seems to vary with each individual ovum. A few typical conditions 
are represented in Figs. 17, 19, 20 and 22. These vesieles may be 
coördinate in size and position or very unequal (Figs. 17, 20). 
In Fig. 17, there is a large and prominent nuclear vesicle in 
the polar cell in a typical nuclear state; while in the narrow part, 
toward the egg, there is a single small vesiele containing a chromo- 
some. No evidence of linin fibres could be distinguished, there was, 
however, a slight stain notieable in the contents of the vesiele 
between the wall and enclosed chromosome. I believe this to be 
