Stockard : Nectar-glands of Vicia Faba 255 



as are found in other parts. The chromatin is arranged much as 

 in the three former cases : in some nuclei the granules are closely 

 packed, in others loosely. 



The cytoplasm now becomes denser and more coarsely gran- 

 ular, staining more heavily than in the three former glands. The 

 nucleus is again, as a rule, surrounded by a dense cytoplasmic ar- 

 rangement. The granules of the general gland-cells take the 

 plasma-stains, while many in the nectar-hairs stain with the 

 nuclear dies. 



(e) Newly matured glands, slightly older than the above. 

 The nucleus is still vacuolated about the plasmosomes. The 

 chromatin is granular and arranged much as above described, in 

 some instances being closely packed and in others thinly. Some 

 nuclei in the lower cell-layers take the plasma-stains, seeming to 

 have lost their chromatic character. The nuclear positions tend 

 toward the cell- center (figure io). 



The cytoplasm is now rather densely granular, the granules of 



of 



pat 



of 



(/) 



The nuclei of these glands 



are slightly shrunken and lie against the cell-walls (figure 17). 

 The vacuole about the plasmosome is not so large and in some is 

 absent ; the granular chromatin is as a rule closely arranged. 

 The nuclei in adjacent tissue-cells are often very small, sometimes 

 apparently absent, and in many cases extremely elongate in form. 

 In fact there is a slight difference in all stages between gland-cell 

 nuclei and general tissue nuclei, the former being as a rule, but 

 not always, larger and tending more toward the spherical form. 



The cell-contents is at this time particularly interesting in its 

 structure, and in all cases is granular in appearance. The cells of 

 the epidermal and lower layers stain very palely with plasma- 

 stains, while the nectar-hairs stain deeply with nuclear dies such as 

 methyl green, iron haematoxylin, and others. In some cases the 

 granules of the cell stain so densely as almost to hide the nucleus 

 which responds to the stain in exactly the same manner as do 

 these granules (figure 17). The contents of the basal cell of the 

 conical hairs takes the plasma-stains, thus differing in its staining 

 reaction from the nectar-hair cells. 



