228 MorGAN: POLARITY AND REGENERATION IN PLANTS 
denuded stalk was left standing in place in connection with its 
original roots. In the course of about ten days new buds began 
to grow out near the base of the stalk. They 
unfolded rapidly and at the end of about two 
weeks had reached the condition shown in the 
figure. The new shoots arose in or near the 
angles between the main stem and the lateral 
branches (which had been cut off). These 
branches stand in the axils of the lower 
leaves. No shoots at all appeared in the 
upper regions of the stem, although the latter 
remained green and in good condition. It 
may appear that the results in this case are 
connected with the attachment of the stem 
to the old roots. The fact that large branches 
arise near the base of the main stem may be 
interpreted to mean that these parts receive a 
large share of the substances that come up 
from the roots. In order to see if this sug- 
gestion had any value I cut off other stems 
from their roots, stripped them of their leaves 
and branches, and placed the lower ends in 
dishes of water. In these cases also the lower 
buds alone unfolded and none appeared in the 
upper parts of the stem. 
To determine whether the result is due to ! 
the better development of the buds at the | 
base rather than due to the movement towards 
the base of food or of so-called formative sub- 
stances, I cut a few of the stems into three 
pieces. In those in which any development 
took place this occurred in the basal pieces, 
and in one case in the lower part of the second 
piece as well, but no buds developed in the 
distal pieces, although to all appearances these 
remained in good condition. Whether these _ 
distal pieces would after a much longer time (I kept them nearly | 
¢- 3 i - 
a Sey tere Pe 02a eens _— 
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three weeks) have developed buds, I do not know. 
