476 Repoiit of the Mycologist of the 



strate this to his satisfaction by placing the cut end of the stem 

 of a white carnation in a bottle of thin red ink. In a short time 

 the white petals will be streaked wiih red. This treatment 

 brings the fungicide into direct contact with the rust-mycelium 

 and we anticipated striking results. 



The first step in the experiment was to determine what effect 

 the treatment would have upon the cuttings themselves. The 

 fungicides used were copper sulphate solution, potassium sul- 

 phide solution, and salt solution. In the tests of spore germina- 

 tion these fungicides had been shown to be capable of preventing 

 the germination of rust uredo-spores. While it does not neces- 

 sarily follow that they would kill the rust-mycelium when 

 brought into contact with it, it is reasonable to suppose that 

 such might be the case. 



In all of the soaking experiments each lot of cuttings was tied 

 into a bunch by itself and the cut ends immersed in the fungicide?. 



The following is a record of the experiments made to determine 

 the effect on cuttings of soaking them in fungicides: — 



Copper sulphate. — eTanuary 23, 1895, ten lots of cuttings were 

 prepared. Each lot contained 25 cuttings — 12 of the variety 

 Michigan and 13 of the variety Uncle John. Three lots were 

 soaked in copper sulphate solution of the strength 1-2000, throe 

 lots in copper sulphate, 1-1000, three lots in copper sulphate, 

 1-500, and one lot, used a check, was not soaked at all. Of the 

 three lots in each of three strengths of copper sulphate, one lot 

 was soaked one-half hour, one lot one hour and the third lot 

 two and one-quarter hours. After soaking, the cuttings were all 

 *' struck " January 23. The results are shown in the following 

 table: 



