—— 62 
farm and as there was no communication 
between it and neighbouring farms it did 
not spread further. 
The ox was then bougth by the Experi- 
ment Station andits serum used on two lots 
of pigs bred near the slaughter-house. 
It proved to be preventive in doses of 
40 cc. for adults and 20 cc. for suckiug pigs. 
It was also used on two Large Black sows, 
in an advanced state of pregnancy, which 
suffered from the disease and had fever, 
varying from 41 to 420 C., with intense 
dyspnoea and were obliged to keep standing, 
with their heads low and their feet well 
apart. On the day following injection, the 
two sows showed no signs of the disease 
by which they might have been distinguished 
from the normal ones. 
The serum had however no effect on 
pigs that already had aphthae and the disease 
ran its usual course. 
A small quantity of the same serum 
was used with good results on some calves 
in Ubá, but as there were only a few of 
them we did not take them into account. 
Other experiments in immunising ani- 
mals were made near Juiz de Fora on 
a ranch belonging to Dr. HERMENGILDO 
VILLAÇA, to whom we are much indebted 
for his kindness. In this place the mortality 
among pure-bred Schwitz calves was terri- 
ble; five of them were dying when we 
arrived. We succeeded in immunising a cow 
from a neighbouring farm, on whichtne epi- 
demic had spent itself. Ten days after the 
last injection, that is a month from starting 
of the work, the disease died out on the 
farm so that we had no further opportuni- 
ties of testing the value of the serum obtained. 
We then transferred the serum to JOAO 
PINHEIRO, in the West of Minas Geraes 
and inoculated five calves which were taken 
from a farm where the infection was recent 
and severe. Another calf, taken from the 
same farm was used as witness; it fell ill 
after three days, had chills and a lot of aphthae 
in its mouth, whereas the calves that had 
been vaccinated did not contract the disease 


during the epidemic, which lasted four 
weeks, 
Dr. HONORIO HERMETO also applied 
the serum in the same place with the same 
results. 
On dosing the serum in our laboratory 
we found that 120 cc. protected adults, 
while 80 cc. were enough for calves over 
six months of age. 
The efficacity of serum obtained by the 
injection of emulsion of aphthae during one 
month at least being proven we were prepa- 
red to make large quantities of it on the 
farms where epidemics occurred. 
Just about this time several farmers ap- 
pealed tothe Minister of Agriculture so as to 
have the necessary measures taken against the 
disease. The work was entrusted by the then 
Minister, PEREIRA LIMA, to Dr. ALCIDES 
MIRANDA, the Chief of the Board of Pas- 
toral Industry, who was given a government 
subvention for the carrying out of the same. 
Our assistant Dr. FRANKLIN DE AL- 
MEIDA made several liters of serum in the 
state of Rio de Janeiro, while we began to 
take prophylactic measures in the state of 
Minas Geraes; the serum was made in 
Uba and usedin Vigosa, Teixeiras aod Ponte 
Nova. 
The technique used was as follows: 
1. Vaccination of well-developed head 
of cattle by rubbing aphthae on lips and ton- 
gue and sub-cutaneus inoculation of 100 
c. c. of serum, 12 hours latter. 
2. Intravenous injection of the emulsion 
of 2,5 gr. of aphthae, 6 days after the first 
injection. 
3. Intravenous injection of the emulsion 
of 10 gr. of aphthae, 12 days after the first 
injection. 
4, Intravenous injection of the emulsion 
of 10 gr. of aphthae, 18 days after the tirst 
injection. 
5. Intnavenous injection of the emulsion 
of 20 gr. of aphthae, 24 days after the first. 
6. Letting of 4 liters of blood from the 
jngular vein, 10 days after the last injection. 
7. After each bleeding, inducement of 
hyperimmunity by injection of 10 gr of fresh — 

