Bee SG Ree 
aphthae, crushed in boiling water and filte- 
red, firstthough cloth and afterwards through 
filtering paper. The animals were always 
bled 10 days after inoculation. 
LOEFFLER warns against the use of 
sputum and other contaminated products in 
hyper immunising. As it is very difficult to 
draw the liquid aseptically and filtering 
through porcelainreduces virulence, we were 
hardly ever able to work with virus from 
natural infection. In a long series of injec- 
tions LOEFFLERused for the most part peri- 
cardial liquid and only seldom aseptically 
taken aphthae, a fact which lenghtened the pe- 
riod for obtaining serum. When the aphthae 
themselves are used, as was the case with 
us, there is a great deal of spontaneus in- 
fection which also lasts longer than that 
obtained from intact boils. Besides this the 
abundance of material allowed us to stren- 
ghten the immunity in a relatively short 
time. It is needless to exagerate the danger 
of contamination by this process as it is not 
of much account, since cattle become immu- 
nised against very virulent germs and much 
more so against the ones generally found 
in the mouth. 
The value of the results obtained can be 
deduced from the statistics we give. As we 
were satisfied with these obtained in 1918, 
we continued our work in 1919, with the 
following results: 
In 1916, at the request of Count MO- 
DESTO LEAL, we set to work on his farm 
in Morro Agudo, where there were cases of 
foot and mouth disease. Thirty nine head of 
cattle were inoculated by one of our assistants 
and lef* with the sick cattle; they did not 
contrac: the disease. 

We then attended to the request of Co- 
lonel ANNIBAL LOPES in Ipiabas, where 
forty. five head were inoculated and also 
left with the sick cattle without having aph- 
thous fever. 
At the same time another member of 
our party inoculated the cattle on a farm be- 
longing to Captain LAUDELINO DA SILVA 
in Pinheiro. Unfortunately there was not 
enough serum to go round; of the eighty 
nine head vaccinated, nine had the disease 
some days after the injections were made. 
In fact we attended to many requests 
and obtained satisfactory results. The follow- 
ing table shows the advantages of traetment 
with anti-aphthous serum. In 80 0/o of the 
cases indemnity was obtained. If we exclude 
the animals that fell sick 48 hours after vac- 
cination, the general percentage rises almost 
to 100 0,0. 
On a ranch in S. Vicente Ferrer, which 
belongs to Mr. C. E. DE AZEVEDO, two 
hundred and seventy head of cattle were 
vaccinated; according to the owner they all 
fell sick. The same thing happened in S. 
Manoel amongst two herds. The serum had 
been obtained by bleeding oxen that did 
not resist immunisation and died several 
days after being bled. This kind of accident 
often happens to novices and hasty workers 
who do not filter carefully enough. Under 
these conditions septic particles get caught 
in the capillaries of the lungs and form 
centers of suppuration. When this occurs, 
the cattle become thin and feverish, cough 
a good deal and generally die on the twelfth 
day after vaccination. 
