16 



l.-lnj. 5 minutes after virus (rubbed in on the head) Did not suffer 



2.- « 10 « « « * * * * t (death) 



3.- « 15 * « « * * * "■ "^ 



4. - « 20 » « « * * • * "^ 



5.- « 25 - - « « « « * t 



6.- Inoculated only with the material from skin lesions (bouba) t 



Only the chick injected 5' after the virus did not suffer, all the others died 

 within 33 days. Chicks 4 and 5 had enormous skin localisaüons (boubas) 

 the remainder smaller ones. 



2nd Scrlca. 

 Chicks one month of ai?e inoculated with 3 cm^ of antiepitheliomatous se- 

 rum and spleen extract. 

 7 - Inj 15 minutes before virus (rubbed in on the head) Nothing 



S^-Id.' ^^ 



9. -Inj. 15 minutes after virus Id 



10.- Id I 



11. Inj, 1 hour after virus T 



12.-ld t 



13.- Inj. of spleen extract one hour afterwards T 



14.-Id I 



15. -Inoculated only with virus f 



16.- Id '^ 



Chicks having been subjected to injection before the inoculation of virus 

 (rubbed in on the head) did not get infected; even injection 1/4 hour after 

 inoculation would only protect one of the two chicks inoculated; from this 

 point on the protective activity was non-existant. Chicks 11 to 14 only sho- 

 wed diphtheric patches, the remainder skin localisations (boubas). 



3rd Scries. 

 Chicks two months old inoculated with 4 cm3 of antiepitheliomatous or 

 normal serum. 

 17.- Inj. 2 hours before (2 cm^) and 1 hour before (another 2 cm3) Did not suffer 



isi-ld.' '^ 



19. ^ Id with normal chicken serum skin localisation (Bouba) 



2(X - Id. Id ^'^'" localisation (Bouba) 



21. -Inoculated only with virus (rubbed in on the head)., skin localisation (Bouba) 



In this experiment also the senun showed an absolute value as a preven- 

 tive. 



Fonrth Scrlei 



Chicks of a little more than two months of age inoculated with 2,4 and 

 cm3. of antiepitheliomatous or normal serum. 



PreventiTC Injections (Antiserum). 



22 -Inj of 2 cni3 24 hours before virus (hypod. inj.) skin localisation (Bouba) 



23" _ Id Did not suffer 



24. - Inj. of 2 cra3 24 and 21 hours before virus (Total 4 cm3) Did not suffer 



