18 



lue of lhe serum was complele, when 

 the serum was used in the dose of 4 to 6 

 cm^. 



We may therefore conclude that se- 

 rum ajiaiiist the epithelioma of fowls 

 may be easily obtained provided that; 

 1" the superimmunised fowls support 

 the inoculations without morbid 

 manifestations, even slight ones 

 (chronic coryza, for instance). 

 2° the activity of the immunising 

 serum be verified with high doses 

 with regard to the weight of the 

 animal that is to be protected aga- 

 inst infection. 



i 



The simple passage of the virulent 



material through musliu and filter paper 

 might, at first sight, seem to prejudice 

 the experime but I had no inten- 

 tion of demonstrating in any way the 

 filterability of the germ o! the diphtheria 

 of fowls; this had already been brillian- 

 tly done by great research- workers; my 

 purpose was to show that fowls super- 

 immunised with virulent material fur- 

 nish serum against a disease produced 

 by a filterable germ that seemed to 

 elude the general principal that; The 

 filterable germs produce, as a rale, pre- 

 ventive serum, when inoculated in a 

 proper manner in animals sensitive to 

 the disease which theif provoke. 



Bello Horizonte, May 21rst, 1922. 



