41 



ice. 16th day-Precip. serum. — Control do- 

 sis. 



4th guinea-pig — 1/1000 anliser. — 2 

 cc. 21lh day-Precip. .serum.— Control do- 

 sis. 



5th guinea-pig — 1/1000 anliser. — 2 

 cc. 26th day-Precip. .serum.— Control do- 

 sis. 



6th guinea-pig — 1/1000 anti.ser. — 2 

 cc— physiol. salt, solution. 



On the second day after inoculation 

 the 3rd, 4th and 5th guinea-pigs died of 

 tetanus; the remaining three kept through 

 out in good health. From this may be 

 deduced that the precipitins capable of 

 removing the antitoxin, only make their 

 appearance towards the 16th day after 

 inoculation, irrespectively of the preci- 

 pitating titre of serum or of the amount 

 of precipitate formed. 



A rabbit was given three subcuta- 

 neous inoculations o'i normal horse se- 

 rum in the following order: . 



On Jan. 10th, 1920-15 cc. 



On Jan. 12th, 1920—10 cc. 



On Jan. loth, 1920 — 10 cc. 



Bled on the 21rst it demonstrated 

 precipitins up to 1/100.000 and with 

 abundant precipitation up to 1/1000 of 

 precipitinogen. 



On Jan. the 23 1920. 



Irst Guinea-pig — 1/1000 anti,ser. — 2 

 cc. Prtcip. serum.— Control dosis. 



2nd Guinea-pig — 1/2000 antiser. — 2 

 cc. Precip. serum.— Control dosis. 



3rd Guinea-pig — 1/1 000 antiser. — 2 

 cc. physiol. salt. sol. Control dosis. 



4th Guinea-pig — 1/2000 antiser. — 2 

 cc. physiol. salt. sol. Control dosis. 



4th guinea-pig died within 60 hours; 

 2nd with 72. The remaining two survi- 

 ved. 



A rabbit vaccinated with concentra- 

 ted antitetanic serum (U. S. Standard), 

 by means of three inoculations, at five 

 days interval each time, was bled 24 

 days after the last injection; it's serimi 



precipitated the antitoxin as well as the 

 ones previously used. 



With diphteria antito.xin, however, 

 in spite of all our efforts we were not 

 able to verify the disappearance of an- 

 titoxin by the use of precipitating sera, 

 although we had used antitoxic sera ob- 

 tained from different sources and of suf- 

 ficiently elevated titre to unable their 

 being employed for titration in as high 

 as those in used in the case of tetanus 

 antito.xin; and although all the precipi- 

 tating sera had their activity againat 

 tetanus antitoxin controlled. 



Guinea-pig of 250 grammes. 



Irst Guinea-pig — 1/700 antidiph. se- 

 rum. — 2 cc. 21th day Precip. S.-Cont. 

 dosis. 



2nd Guinea-pig — 1/1000 antidiph. se- 

 rum. — 2 cc. 21th day Precip. S.-Cont. 

 dosis. 



3rd Guinea-pig — 1/700 antidiph. se- 

 rum. — 2 cc. phys. Salt. sol. S. — Cont. 

 dosis. 



4th Guinea-pig— 1/1000 antidiph. se- 

 rum — 2 cc. phys. Salt. sol. S. — Cont 

 dosis. 



Of these guinea-pig the last one died 

 between the fourth and the fifth day, 

 the second one five hours afterwards, 

 both of them with congestion of adrenals 

 and hemorrhagic Infiltration at the site 

 of inoculation. 



250 gr. guinea-pigs. Precipitating se- 

 rum of 36 days. 



Irst guinea-pig — 0,1 standard antis. 



— 2cc. Precip. S. — 0,7 toxin (control do- 

 sis. 



2nd guinea-pig — 0,1 standard antis. 



— 3 cc. Precip. S. — 0,7 toxin (control do- 

 sis. 



3rd guinea-pig — 0,1 standard antis. 



— 2 cc. phys. s. sol. — 0,7 toxin (control 

 dosis. 



These guinea-pigs died one after 

 another at short intervals on the night 

 between fourth to fifth day. 



Precipitating serum of 30 days. 



