42 



Irst pigeon — 1/800 anli-dipht s.— 2 

 cc precip. S.— Control dosis. 



2nd pigeon — 1/1000 anti-dipht. s. —2 

 (CC. precip. S.— Control dosis. 



3rd pigeon — 1/800 anti-dipht. s.— 2 

 cc. physiol. salt. sol. — Control dosis. 



4th pigeon — 1/iOOO anti-dipht. s. —2 

 cc. physiol. salt. sol. — Control dosis. 



All the animals survived, except the 

 Hast one which died on the 6th day. 



Notwithstanding our eagerness to 

 provide the best condilions for obtaining 

 positive results, we found it invariably 

 impossible to remove the diphteria an- 

 îiloxin by means of anti-horse rabbit se- 

 rum. 



These results explain however the 

 reason why the experiments of KRAUS 

 and PRIBRAM entirely disagree with 

 «lose of DEHNE and HAMBURGER as to 

 ïhe precipitation of antilo.xin in vivo, for 

 while the latter worked with tetanus an- 

 tito.-iin the others employed diphtheria 

 antitoxin in their investigations. For this 

 reason KRAUS and PRIBRAM'S conclu- 

 sions on this subject do not possess an 

 unquestionable value. 



But not only with diphtheria antito- 

 xin did we have negative results; the ex- 

 periments with bothropic antivenin led 

 lus to similar results. These results arc, 

 however, more open to criticism, seeing 

 Ihat for the titration of serum it is not 

 possible to make high dilutions of prc- 

 dpilinogen, which according to K. and P. 

 is essential in obtaining reliable resul- 

 ts. To obviate this drawback, we used 

 excessive quantities of precipitating se- 

 rum which had, as in the case of the diph- 

 iheria antitoxin, been previously vcrilied 

 with tetanus antitoxin. 



The anti venin used was obtained 

 from the Serum-therapeutic Institute of 

 Bulantan, State of São Paulo, and had 

 titration indicated on the basis of 1 ca 

 of serum corresponding to 1,6 of poison 

 of Lachesis lanceolalus. The poison 

 we obtained from Bello Horizonte. 



All the titrations were made by the pro-* 

 cess of VITAL BRAZIL. Before adding 

 the poison the tubes were left with an- 

 titoxic and precipitating serum during 

 2 hours in the incubaton at 37° Centigra- 

 de. After the addition of the poison they 

 remained yet another hour at 37o C. be- 

 fore being injected in the axillary vein of 

 the pigeon. 



Irst pigeon — 1 cc. Bothr. Ser. 3 cc. 

 Precip. Ser.— 1,6 poison Lachesis lan- 

 ceolatus. 



2nd pigeon — 1 cc. Bothr. Ser. 3 cc. 

 Precip. Ser.— 1,6 poison Lachesis lan- 

 ceolalus. 



3rd pigeon — 1 cc. Bothr. Ser. 1 cc. 

 Precip. Ser.— 1,6 poison Lachesis lan- 

 ceolalus. 



4lh pigeon — 1 cc. Bothr. Ser. 3 cc. 

 dist* water — 1,6 poison Lachesis lanceo- 

 latus. 



5th pigeon — 1,6 of poison of La- 

 chesis lanceolatus. 



All the pigeons kept in good health 

 and survived the inoculation, except the 

 last one which died immediately. 



2nd Series: 



Irst Pigeoon — 1/10 Bothr. serum 2 

 cc. Precip. S. — 0,16 poison L. lanceo- 

 latus. 



2nd Pigeon — 1/10 Bothr. serum 1,5 

 Precip. S. — 0,16 poison L. lanceolatus. 



3rd Pigeon — 1/10 Bothr. serum Ice. 

 Precip. S. — 0,16 poison L. lanceolatus. 



4lh Pigeon — 1/10 Bothr. serum 2cc. 

 phys. s. s. 0,16 poison L. lanceolatus. 



5th Pigeon — 0,16 poison of Lachesis 

 lanceolatus. 



All these animals resisted without 

 alteration of their state of health ex- 

 cepting the last one which died within 

 five minutes. 



The scarcity of the serum in the 

 market prevented us from performing 

 other experiments and from determining 

 titrations with Crotalus (rattle-snake) an- 

 tivenin The precipitating serum emplo- 



