43 



yed in these experiments was oí 24 days. 



Still more interesting were the re- 

 snlts obtained with tetanus antitoxin and 

 human anti-horse precipitating serum. 



AS. received in two days 300 cc. of 

 antipest serum in three injections, two 

 in the vein and one subcutaneously. Last 

 Injection on Nov. 11th, 1919. 



On Nov. 19th, 1919 he was bled 

 and the serum demonstrated: 



Presence of precipitinogen up to a 

 dilution of 1/10. 



Presence of precipitins up to a dilu- 

 tion higher than 1/1000. 



Absence of precipitin for tetanus an- 

 titoxin. 



On Nov. 26, 1919, bled once again 

 his serum demonstrated: 



Presence of precipitinogen up to 

 1/10. 



Presence of precipitins up to 

 1/1000. 



Complete absence of precipitins for 

 tetanus antitoxin. 



On Dec. 14, 1919, bled for the last 

 time, the patients scrum upon being ana- 

 lysed demonstrated: 



Presence of precipitinogen up to 

 1/2. 



Presence of precipitins up to 

 1/10.000 



Absence of precipitin for tetanus an- 

 titoxin. 



C. R. received 320 cc, antipest serum 

 in three intravenous inoculations, the 

 last one on Nov. 11, 1919. 



On Nov. 22, 1919, after bleeding the 

 serimi showed: 



Presence of precipitinogen up to 

 1/1 a 



Presence of precipitins up to 

 1/1000. 



Absence of precipitin for tetanus 

 antitoxin. 



On Dec 5, 1919. 



Presence of precipitinogen up to 

 1/2. 



Presence of precipitins up to 

 1/1000. 



Absence of any action upon tetanus 

 antitoxin. 



On Dec. 29, 1919. 



Absence of precipitinogen. 



Presence of precipitins up ta 

 1/1000. 



Absence of any harmful effect for 

 tetanus antitoxin. 



The precipitinogen was searched for 

 with the aid of anti-horse precipitating 

 rabbit serum, of 17 days. The titrations 

 of the antitoxin were performed with 

 such an approximation to the antitoxic 

 titre of serum that a distinct local teta- 

 nus was obtained in the guinea-pigs. 



Later on, we had opportunitity of 

 examining three more individuals, but 

 we exempt ourselves from exposing the 

 results in detail, as they do not in any 

 way differ from the ones mentioned. In 

 no case could we obtain a result that 

 might iu any way be considered as doubt- 

 itful. 



This demonstrates, therefore, that the 

 precipitinogens for certain animal spe- 

 cies may not act as such on other, ones» 

 although these may react with precipitins 

 on other fractions of the serum em- 

 ployed. 



Studies on rabbits, with dysentery 

 antitoxin gave us completely different 

 results. 



Teclinic; 



We placed 0,1 of anli-Shiga seriun, 

 concentrated 6 times, together with pre- 

 cipitating serum of 16 days, of 4 days, 

 and normal rabbit serum for two hours 

 at 37o Centigrade. This mixture, after ccn- 

 trifugalion and décantation, inoculated 

 iU the marginal vein of the ear, was fol- 

 lowed after half an hour, and this lime 

 in the vein of the opposite ear, by an 

 inoculation of 0,4 of Shiga extract. 



Irst Rabbit — 1 cc. 16lh day ser. — 

 0,1 Shiga s. — 0,4 Shiga extract. 



2nd Rabbit — 1 cc. 4th day ser. — « 

 0,1 Shiga s. — 0,4 Sliiga extract 



