46 



Bled on Jan. 9, 1920, this animal 

 furnished antitoxic serum with 500 A. 

 U. per cubic centimetre and precipita- 

 ting up to 1/100. 



On Jan. 15, 1920, to 0,5 of the serum 

 of this horse sheep serum diluted at 1/10 

 was added. After two hours at 37oC. and 

 24 hours in the refrigerator it was cen- 

 trifugated and the liquid poured off was 

 diluted in such a manner that the antito- 

 xic serum became diluted at 1/1.000, 

 1/2.000, and 1/5.000. Controls accompa- 

 nied this process with physiologic sali- 

 ne solution instead of sheep serum. To 

 each dilution was added the control 

 dosis and after an hour's interval the 

 inoculations were made on guinea pigs 

 of 350 grammes. 



Irst 

 titox. s. 



2nd 

 titox. s. 



3rd 

 titox. s. 



4th 

 titox. s. 



5th 

 titox. s. 



6lh 

 titox. s. 



g-p- 



— 2 cc 



g-P- - 



— 2 cc 



g-P- - 



— 2 cc 



g-p.- 



— 2 cc 



g-p.- 



— 2 cc 



g-P- 



— 2 cc 



an- 



an- 



an- 



1/1000 precipitated 

 phys. s. Cont. dos. 

 1/2000 precipitated 

 phys. s. Cont. dos. 

 I/ÕOOO precipitated 

 phys. s. Cont. dos. 

 1/1030 non precipitated an 

 . phys. s. Cont. dos. 

 1/2C03 non precipitated an 

 . phys. s. Cont. dos. 

 1/5000 non precipitated an 

 . phys. s. Cont. dos. 



The third and the sixth guinea pigs 

 died of tetanus on Jan. 20; the remai- 

 ning four survived. 



A goal was inoculated on: 



Oct. 28, 1919 with 50 cc. of toxoids. 



Nov. 7, 1919 with 100 cc. of toxoids. 



Nov. 17, 1Ü19 with 100 cc. of toxoids. 



Nov. 27, 1919 with 150 cc. of toxoids. 

 • Dec 10, 1S19 with 150 cc. of toxoids. 

 f Dec. 20, 1919 with 20 cc. of toxin. 



Dec. 30, 1919 with 40 cc. of toxm. 

 ' Jan. 9, 1920 with 50 cc. of toxin. 



Jan. 19, 1920 with 50 cc. of toxin. 



Jan. 20, 1920 with 100 cc. of normal 

 faorse serum. 



Jan. 29, 1920 with 50 cc. of toxin. 



Jan. 30, 1920 with 100 cc. of normal 

 horse serxim. 



This animal was bled on Feb. 2, 

 1920; the serum contained 50 U. A. per 

 cubic centimetre and precipitated up to 

 1/1 OOO 



The technic used in this case was 

 exactly equal to the preceding one, the 

 serum was precipitated by a dilution at 

 1/10, the precipitate being more abun- 

 dant in this tube. 



Irst guin. pig.— 1/50C precipitated ant. 

 s.— 2 cc, phys. sal. sol. control dosis. 



2nd guin. pig.— 1/1000 precipitated 

 ant. s. — 2 cc. phys. sal. sol. control dosis. 



3rd guin. pig.— 1/500 non precipita- 

 ted ant. s.— 2 cc. phys. sal. sol. control 

 dosis. 



4th guin. pig.— 1/1000 non precipita- 

 ted ant. s.— 2 cc. phys, sal sol. control 

 dosis. 



Second and fourth guine.i pigs died 

 in the course of the second day, third 

 one diu*ing the afternoon of the fourth 

 day and first one was found dead on 

 the fifth day. 



Thus here also one sees that the 

 removal of the fraction of the serura 

 which constitutes in them their precipi- 

 tating part is entirely independent of the 

 part formed by antitoxin in animals of 

 a different species, The removal of this 

 part does not give the sera higher anti- 

 toxic powers nor greater affinity forth* 

 toxin. 



Rio, March the 12th, 1920 



