96 



shaken and finally the remaining 4 cc. 

 are added. 



The aqueous sohilion of all the 

 antigens l)eing ready, there only remains 

 to mix it with the positive and nega- 

 tive sera. The hlood sera should he 

 diluted with physiologic solution at 0,85 

 o/o in the proportion of 1 to 10. To I cc. 

 of these serum dilutions 0,5 cc. antigen 

 is addctl. The same process is used for 

 all the antigens, mixing them with po- 

 sitive and negative sera. 



The tuhes are kept in the incuhator 

 at 37"(; during 21 hours, at the end 

 of which time the results are read. 

 One notes the tuhe in which floccula- 

 tion is most intense and then one oh- 

 serves if the negative sera remain unal- 

 tered with the same antigen. In that 

 caso the right antigen is delermined 



and there only remains to note the titre 

 of cholesterin used. 



Titration should he made with ma- 

 ny sera, both positive and negative, in 

 order to know the antigen well ])efore 

 it is currently used. 



We give a table, by which the Ic- 

 chnic just described is easily understood. 

 It refers to one extract only and also to 

 one positive and one negative serum. Ano- 

 thei- table gives the test of antigen with 

 physiologic solution only, for control- 

 ling if tlocculalion without presence of 

 serum occurs. 



.^ny other extract is titrated in the 

 same way. I constantly obtained excel- 

 lent results by following this Icchnic 

 cUid have used it in 2000 cases uniler 

 I he W.\SSERMAX\ lest. 



Tubes 



Titatrîon 



2 3 



I control by 

 of Antigen 



4 5 6 



10 



Concentrated Antigen 

 extact. 



Absolute alcohol. 



Alcoholic cholesterin 

 solution iQ/o. 



1 cc. 



I cc. 



1 cc. 



i 2 cc. 2 cc. 2 cc. 



0,1 



1 cc. 1 cc. 1 cc. 



1 cc. 



2 cc. 



0,2 



0,3 0,4 



2 cc. 



2 cc. 2 cc. 



0,5 0,6 



0,7 



1 cc. 



2 cc. 



O.S 



1 cc. ! 1 cc. 



2 cc. 



0,9 



2 cc. 



1 cc. 



I Dilute the contents of each tube with physiologic saline solution 

 i at 0,85"/o in the proportion of 1 to 6. 



Diluted extracts. i 0,5 | 0,5 ' 0,5 0,5 0,5 | 0,5 I 0,5 i 0,5 ! 0.5 | 0,5 



Positive serum at l/lO. i 1 cc. ! 1 cc. ' 1 cc. , 1 cc. • 1 cc. 1 cc. i 1 cc. ¡ 1 cc. 1 cc. 1 cc.^ 



24 hours in the incubator at 37oC. 



Diluted extracts. i| 0,5 



0,5 



Negative serum at 1/10. 1 1 cc. 1 cc. 



0,5 



1 cc. 



0,5 



0,5 



0,5 



0,5 I 0,5 



0,5 



1 cc. 1 cc. 1 1 cc 1 cc. I 1 cc. I 1 cc. 1 cc. 



24 hours in the incubator at 37oC. 



0.5 



