97 



Saline Sol. at 

 0,85o u 



Diluted extracts 



1 cc. 1 cc. 1 cc. 



0,5 0,5 0,5 



1 cc. 

 0,5 



Technîc for the use of the reaction 



Having obtained the titraled anti- 

 s^cns it is easy to perform the reaction 

 The antigen is prepared by diluting 

 the concentrated solution in two parts 

 of absolulc alcohol and adding the quan- 

 tity of cholesterin determine^ by titration. 

 The next step is to make the dilution 

 of the anligen in the proportion of 1:6, 

 iis explained above. The serum for exa- 

 mination is diluted with ten parts of 

 physiological solution and to 1 cc. of 

 the resulting mixture 0,5 antigen is nd 

 ded. Known positive and negative sci-:i 

 are used as checiis and the test is made 

 with all of them. The tubes are kept in 

 the incúbalo!' at 37^C. for 20 hours wlien 

 the results are noted. The temperature 

 must I'cmain constant.) 



In the accompanying table lhe three 

 extracts and the whole lechnic are re- 

 gistered. 



The best way lo read results consists 

 in holding the tube against a dark back- 

 groiuid, so as to ¡jerceive the smallest 

 flocculae. 



The I'esults are classified b/v the 

 more or less intense flocculation using 

 the following signs:-] — \ — \- when the 

 flocculation is very strong and a deposit 

 of precipitate is formed in the tube; 

 + -|- positive with less precipitate:-!- 

 weakly positive when the precipitate is 

 thin and only i)erceived against a dark 

 background;— negative when there is no 

 precipitate. 



In the statistics published in the 



Portuguese text, page 121 and referring 



to 2000 sera submitted to Sachs-Georgi 



-and \r.\SSERM.\XX tests the above men- 



1 cc. 1 cc. ' 1 cc. 



0,5 0,5 



0,5 



1 cc. 



0,5 



1 cc. 



0,5 



1 cc. 

 0,5 



24 hours in the incubator at 37oC. 



lioned conventional signs are used; fur- 

 thermore, controls are indicated by the 

 word testemunhos and the reactions car- 

 ried out with physiological saline li- 

 quid by the abbreviation: li([. 



The process is the same as for tlie 

 blood serum and for greater sensibitive- 

 ncss the liquid may be used pure. 



SACHS GEORGI Test 



Extr. A. 



Serum to be tested di- 

 luted at 1/10 



Antigens 



Positive serum diluted 



at 1/10 



Antigens 



1 cc 

 0,5 



Negative serum diluted 



at 1/10 



Antigens 



Saline Sol. at 0,85o/o. 

 Antigens 



1 cc. 

 0,5 



1 cc. 

 0,5 



1 cc. 

 0,5 



Extr. B. 



1 cc. 

 0,5 



1 cc. 

 0,5 



1 cc. 

 0.5 



1 cc. 

 0,5 



Extr. C. 



1 cc. 

 0,5 



1 cc. 

 0,5 



1 cc. 

 0,5 



1 cc. 

 0.5 



24 hours in the incubator at 37oC. 



SYNOPSIS OF STATISTICS 



W.\SSERMANN TEST positive I 



SACHS-GEORGI test positive ) 



W.VSSERMAXX test negative | 

 SACHS-GEORGI lest negative ) 



684 



1,178 



WASSERMAXX lest positive I 



74 

 S.VCHS-GEORGI lest negative | 



