Experiments on the Respiration of Melampus. 285 
Experiment 9. 
June 1. Two specimens of Melampus equalis, from the same place, 
were inclosed in separate bags, and immersed in the same 
glass of sea-water. 
—— 12. - Both were dead ; the water never having been changed since 
June the 4th, when they were certainly alive. They were also 
alive on either the 6th or 7th, but I cannot speak quite 
positively. 
Experiment 10. 
June 22. Twospecimens of the same inclosed and immersed as above. 
26. Both dead. The water has not been changed. 
Experiment 11. 
June 26. Two specimens of the same inclosed and immersed as before. 
— 29. One dead ; the other sickly. The water was changed.* 
30. The survivor alive. 
July 1. Dead. 
Experiment 12. 
July 19. Two specimens of the same inclosed in bags, and immersed 
in separate glasses of sea-water. 
—— 20. Twenty-four hours after both are alive and healthy ; thirty 
hours after, one is sickly and retracted; the other quite 
healthy. 
—— 21. The last is quite healthy; the other quite retracted, and, I 
think, dead. Water changed for both. 
—— 22. Both quite dead. 
Experiment 13. 
Aug. 10. Two specimens of the same inclosed in separate bags, were 
immersed in the same glass of sea-water. 
N.B. These are the two specimens mentioned in Experiment 8. 
After the air had been expelled in the manner there described, they were 
left covered by the water; no more air-bubbles appeared. 
* The water was changed in this, and in all theexperiments, by pouring in 
gently the fresh, and suffering the contents of the glass to run over its sides till 
the water was completely renewed. Thus, no part of the bags was ever for an 
instant exposed to the air. Care must be taken to pour in the water gently 
lest bubbles of air should be driven into the bags; which should also be well 
soaked previously to the experiment, to expel every particle of the same. 
