THE BROWN ROT OF SOLANACEAE I TECHNIC 



189 



and must continue to grow rapidly for at least several weeks. 

 Only disappointment will result from inoculations on old slow- 

 growing, woody plants, or with cultures which have been in the 

 laboratory for a considerable period. The best results may 



Fig. 131. — Common sunflowers (Nos. 3 and 4) inoculated- with Bacterium 

 solanacearum by needle pricks on July 12, 1918, at X (on the stem) and badly 

 dwarfed and wilting. The controls at either side were 33 inches high. Photo- 

 graphed July 30, 1918, about ,^ natural size. Experiments of Smith and Godfrej'. 



be expected from cultures isolated the same summer they are 

 used. 



Determine 



1. For THE ORGANISM. Morphologij. — Size in microns (stain 

 with methyl violet or Ziehl's carbol fuchsin), form, aggregation 



